ArticleThe EMBO journal1996
Context dependent transactivation domains activate the immunoglobulin mu heavy chain gene enhancer.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Gcn5 is required for PU.1-dependent IL-9 induction in Th9 cells.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF.The Journal of biological chemistry · 2009Article
- A dimer-specific function of the transcription factor NFATp.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish.Nucleic acids research · 2008Article
- Ets target genes: past, present and future.Oncogene · 2000Review
- Transcriptional activation by ETS and leucine zipper-containing basic helix-loop-helix proteins.Molecular and cellular biology · 1999Article
- Exploring functional redundancy in the immunoglobulin mu heavy-chain gene enhancer.Molecular and cellular biology · 1998Article
- Normal myeloid development requires both the glutamine-rich transactivation domain and the PEST region of transcription factor PU.1 but not the potent acidic transactivation domain.Molecular and cellular biology · 1998Article
- Multiple functional domains of AML1: PU.1 and C/EBPalpha synergize with different regions of AML1.Molecular and cellular biology · 1998Article
- ETS-core binding factor: a common composite motif in antigen receptor gene enhancers.Molecular and cellular biology · 1998Article
- ETS-mediated cooperation between basic helix-loop-helix motifs of the immunoglobulin mu heavy-chain gene enhancer.Molecular and cellular biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enhancers and promoters nucleate the assembly of multiprotein complexes that are required for the transcriptional activation of eukaryotic genes. Although multimerized binding sites of individual transcription factors sometimes mimic the properties of an enhancer, the combinatorial use of factors is considered to be crucial for achieving biological specificity. The minimal B cell specific immunoglobulin mu heavy chain gene enhancer is activated by a combination of tissue-restricted ETS proteins and ubiquitously expressed basic helix-loop-helix transcription factors. Here we show that a domain of PU.1 that activates transcription from multimerized PU.1 binding sites is not required to activate the mu enhancer together with Ets-1. In contrast, a transactivation domain in Ets-1 is necessary to activate this enhancer synergistically with PU.1. Furthermore, the Ets-1 activation domain functions only when tethered to the muA site of the enhancer. These observations illuminate two forms of context dependence: first, all possible transcription activation domains may not be required to achieve combinatorial specificity; second, functional transcription activation domains may require appropriate positioning on DNA.
Indexed as
Identifiers
8887557PMC452198W6965533What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.