ArticleThe EMBO journal1996
Distinct activated and non-activated RNA polymerase II complexes in yeast.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 61 citations in OpenAlex.
- The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors.Nucleic acids research · 2010Article
- Patterns of gene-specific and total transcriptional activity during the Plasmodium falciparum intraerythrocytic developmental cycle.Eukaryotic cell · 2009Article
- Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo.Molecular and cellular biology · 2005Article
- Transcriptional coactivator PC4 stimulates promoter escape and facilitates transcriptional synergy by GAL4-VP16.Molecular and cellular biology · 2004Article
- Article
- The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.Molecular and cellular biology · 2003Article
- Yeast and Human RNA polymerase II elongation complexes: evidence for functional differences and postinitiation recruitment of factors.Eukaryotic cell · 2003Article
- CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo.Genes & development · 2002Article
- The regulatory role for the ERCC3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genetic interactions of Spt4-Spt5 and TFIIS with the RNA polymerase II CTD and CTD modifying enzymes in Saccharomyces cerevisiae.Genetics · 2001Article
- TFIIS enhances transcriptional elongation through an artificial arrest site in vivo.Molecular and cellular biology · 2001Article
- Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation.Genetics · 2001Article
- Analysis of gene induction and arrest site transcription in yeast with mutations in the transcription elongation machinery.The Journal of biological chemistry · 2001Article
- Dynamic association of capping enzymes with transcribing RNA polymerase II.Genes & development · 2000Article
- Discrete promoter elements affect specific properties of RNA polymerase II transcription complexes.Nucleic acids research · 2000Article
- hnRNP U inhibits carboxy-terminal domain phosphorylation by TFIIH and represses RNA polymerase II elongation.Molecular and cellular biology · 1999Article
- ICP22 and the UL13 protein kinase are both required for herpes simplex virus-induced modification of the large subunit of RNA polymerase II.Journal of virology · 1999Article
- Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Poly(A) signals control both transcriptional termination and initiation between the tandem GAL10 and GAL7 genes of Saccharomyces cerevisiae.The EMBO journal · 1998Article
- Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast.Genes & development · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used a transcriptional run-on assay in permeabilized yeast cells to study the distribution of RNA polymerase II (pol II) complexes before and after activation by Gal4. Polymerases were found engaged on the gene at the 5' end before activation, but only appeared at the 3' end after activation. Mutations of the pol II C-terminal domain (CTD), the CTD kinase Kin28 and the holoenzyme subunit Srb2 all inhibited the formation of 3' polymerases in response to activator. However, these mutations did not inhibit the establishment of polymerases at the 5' end. The differences between 3' and 5' ternary complexes suggest that they represent qualitatively distinct 'activated' and 'non-activated' forms of polymerase. The results implicate CTD phosphorylation in a switch from 'non-activated' transcription, which is confined to the 5' end, to an 'activated' mode that traverses the length of the gene.
Indexed as
Identifiers
8887556PMC452197W222278129What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.