ArticleThe EMBO journal1996
The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 98 citations in OpenAlex.
- Review
- Analysis of transcriptional response in haploid and diploid Schizosaccharomyces pombe under genotoxic stress.G3 (Bethesda, Md.) · 2024Article
- Polymorphism ofInternational journal of molecular sciences · 2023Article
- Increased expression of Polδ does not alter the canonical replication programWellcome open research · 2021Article
- Review
- Roles of human POLD1 and POLD3 in genome stability.Scientific reports · 2016Article
- POLD3 Is Haploinsufficient for DNA Replication in Mice.Molecular cell · 2016Article
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.Genetics · 2015Review
- Eukaryotic DNA polymerase ζ.DNA repair · 2015Review
- Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5'-flaps.Nucleic acids research · 2015Article
- Initiation of DNA damage responses through XPG-related nucleases.The EMBO journal · 2013Article
- A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis.Nucleic acids research · 2012Article
- Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis.BMC molecular biology · 2009Article
- p8/TTDA overexpression enhances UV-irradiation resistance and suppresses TFIIH mutations in a Drosophila trichothiodystrophy model.PLoS genetics · 2008Article
- X-ray structure of the complex of regulatory subunits of human DNA polymerase delta.Cell cycle (Georgetown, Tex.) · 2008Article
- Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.Molecular biology of the cell · 2008Article
- A functional analysis of PCNA-binding peptides derived from protein sequence, interaction screening and rational design.Oncogene · 2006Article
- An in vivo analysis of the localisation and interactions of human p66 DNA polymerase delta subunit.BMC molecular biology · 2005Article
- A single domain in human DNA polymerase iota mediates interaction with PCNA: implications for translesion DNA synthesis.Molecular and cellular biology · 2005Article
- Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
cdc1+ is required for cell cycle progression in Schizosaccharomyces pombe. Cells carrying temperature-sensitive cdc1 mutants undergo cell cycle arrest when shifted to the restrictive temperature, becoming highly elongated. Here we describe the cloning and sequencing of cdc1+, which is shown to encode a 462 residue protein that displays significant sequence similarity to the small subunit of mammalian DNA polymerase delta. cdc1+ interacts genetically with pol3+, which encodes the large subunit of DNA polymerase delta in fission yeast, and the Cdc1 protein binds to Pol3 in vitro, strongly suggesting that Cdc1 is likely to be the small subunit of Pol delta. In addition, we show that cdc1+ overexpression is sufficient to rescue cells carrying temperature-sensitive cdc27 alleles and that the Cdc1 and Cdc27 proteins interact in vivo and in vitro. Deletion of either cdc1+ or cdc27+ results in cell cycle arrest with the arrested cells having a single nucleus with 2C DNA content. No evidence was obtained for a cut phenotype, indicating that neither cdc1+ nor cdc27+ is required for checkpoint function. cdc1 mutant cells are supersensitive to the DNA synthesis inhibitor hydroxyurea and to the DNA damaging agent MMS, display increased frequency of mini-chromosome loss and have an extended S phase.
Indexed as
Identifiers
8887553PMC452193W1600623344What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.