ArticleThe EMBO journal1996
Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 182 citations in OpenAlex.
- Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast.iScience · 2026Article
- Inhibition mechanism of the fungal β-1,3-glucan synthases by triterpenoid antifungal drugs.Nature communications · 2026Article
- The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.PLoS biology · 2025Article
- Spatial control of secretory vesicle targeting by the Ync13-Rga7-Rng10 complex during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- Fungal β-1,3-glucan synthase: a review of structure, mechanism, and regulation.FEMS yeast research · 2025Review
- Structure of a fungal 1,3-β-glucan synthase.Science advances · 2023Article
- Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.Journal of cell science · 2023Article
- Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells.Open biology · 2023Article
- Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability.International journal of molecular sciences · 2022Review
- The Small Ras Superfamily GTPase Rho4 of the Maize Anthracnose FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond.Journal of fungi (Basel, Switzerland) · 2021Review
- Marine natural products targeting the eukaryotic cell membrane.The Journal of antibiotics · 2021Review
- The Role of the Cell Integrity Pathway in Septum Assembly in Yeast.Journal of fungi (Basel, Switzerland) · 2021Review
- Review
- Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.Molecular biology of the cell · 2020Article
- Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe.Molecular microbiology · 2019Review
- Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions.iScience · 2019Article
- A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.Journal of cell science · 2019Article
- Rho Family GTPases in Fission Yeast Cytokinesis.Communicative & integrative biology · 2019Review
11 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Schizosaccharomyces pombe Cdc42 and Rho1 GTPases were tested for their ability to complement the cwg2-1 mutant phenotype of a decrease in (1-3)beta-D-glucan synthase activity when grown at the non-permissive temperature. Only Rho1 is able to partly complement the defect in glucan synthase associated with the cwg2-1 mutation. Moreover, overexpression of the rho1 gene in wild-type S.pombe cells causes aberrant morphology with loss of polarity and cells with several septa. Under this condition (1-3)beta-D-glucan synthase activity is increased four times, but is still dependent on GTP. When S.pombe is transformed with constitutively active rho1 mutant alleles (rho1-G15V or rho1-Q64L), cells stop growing and show a very thick cell wall with hardly any septum. Under this condition the level of (1-3)beta-D-glucan synthase activity is at least 20 times higher than wild-type and is independent of GTP. Neither cdc42+ nor the cdc42-V12G or cdc42-Q61L constitutively active mutant alleles affect (1-3)beta-D-glucan synthase activity when overexpressed in S.pombe. Cells overproducing Rho1 are hypersensitive to inhibitors of cell wall biosynthesis or to cell wall degrading enzymes. We conclude that Rho1 GTPase directly activates (1-3)beta-D-glucan synthase and regulates S.pombe morphogenesis.
Indexed as
Identifiers
8887550PMC452188W4128910What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.