Evidence map›Paper›PMID 8885236›Full record

ArticleMolecular biology of the cell1996

Activation of the osmo-sensitive chloride conductance involves P21rho and is accompanied by a transient reorganization of the F-actin cytoskeleton.

B C Tilly, M J Edixhoven, L G Tertoolen, N Morii, Y Saitoh, S Narumiya, H R de Jonge

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PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
4.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 171 citations in OpenAlex.

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  9. Hypotonic swelling-induced activation of PKN1 mediates cell survival in cardiac myocytes.American journal of physiology. Heart and circulatory physiology · 2011
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

B C TillyDepartment of Biochemistry, Cardiovascular Research Institute COEUR, Medical Faculty, Erasmus University, Rotterdam, The Netherlands.
M J Edixhoven
L G Tertoolen
N Morii
Y Saitoh
S Narumiya
H R de Jonge
Erasmus MC · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hypo-osmotic stimulation of human Intestine 407 cells rapidly activated compensatory CL- and K+ conductances that limited excessive cell swelling and, finally, restored the original cell volume. Osmotic cell swelling was accompanied by a rapid and transient reorganization of the F-actin cytoskeleton, affecting both stress fibers as well as apical ruffles. In addition, an increase in total cellular F-actin was observed. Pretreatment of the cells with recombinant Clostridium botulinum C3 exoenzyme, but not with mutant enzyme (C3-E173Q) devoid of ADP-ribosyltransferase activity, greatly reduced the activation of the osmo-sensitive anion efflux, suggesting a role for the ras-related GTPase p21rho. In contrast, introducing dominant negative N17-p21rac into the cells did not affect the volume-sensitive efflux. Cell swelling-induced reorganization of F-actin coincided with a transient, C3 exoenzyme-sensitive tyrosine phosphorylation of p125 focal adhesion kinase (p125FAK) as well as with an increase in phosphatidylinositol-3-kinase (PtdIns-3-kinase) activity. Pretreatment of the cells with wortmannin, a specific inhibitor of PtdIns-3-kinase, largely inhibited the volume-sensitive ion efflux. Taken together, our results indicate the involvement of a p21rho signaling cascade and actin filaments in the activation of volume-sensitive chloride channels.

Indexed as

Botulinum ToxinsActinsADP Ribose TransferasesAnionsCell Adhesion MoleculesCells, CulturedChloride ChannelsCytoskeletonFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGTP-Binding ProteinsHumansHypotonic SolutionsIntestinesOsmotic PressurePhosphatidylinositol 3-KinasesActinsADP Ribose TransferasesAnionsBotulinum ToxinsCell Adhesion MoleculesChloride Channelsexoenzyme C3, Clostridium botulinumFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGTP-Binding ProteinsHypotonic SolutionsPhosphatidylinositol 3-KinasesPhosphotransferases (Alcohol Group Acceptor)Protein-Tyrosine KinasesPTK2 protein, humanrho GTP-Binding ProteinsTyrosine

Identifiers

PMID8885236
PMCPMC275991
OpenAlexW2005313327

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.