ArticleMolecular biology of the cell1996
The association of annexin I with early endosomes is regulated by Ca2+ and requires an intact N-terminal domain.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 76 citations in OpenAlex.
- Revisiting the role of Annexins in membrane trafficking.Cellular and molecular life sciences : CMLS · 2025Review
- A comparative analysis of paxillin and Hic-5 proximity interactomes.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Modeling the Annexin A1-S100A11 heterotetramer: a molecular dynamics investigation of structure and correlated motion.Journal of biomolecular structure & dynamics · 2024Article
- The Calcium Binding Protein S100A11 and Its Roles in Diseases.Frontiers in cell and developmental biology · 2021Review
- A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages.PloS one · 2021Article
- Influenza A virus enhances its propagation through the modulation of Annexin-A1 dependent endosomal trafficking and apoptosis.Cell death and differentiation · 2016Article
- Annexin1 regulates DC efferocytosis and cross-presentation during Mycobacterium tuberculosis infection.The Journal of clinical investigation · 2015Article
- Annexin A1 regulates TGF-beta signaling and promotes metastasis formation of basal-like breast cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Proteomic analysis of lamellar bodies isolated from rat lungs.BMC cell biology · 2008Article
- Membrane-induced folding and structure of membrane-bound annexin A1 N-terminal peptides: implications for annexin-induced membrane aggregation.Biophysical journal · 2008Article
- Annexin A6-induced alterations in cholesterol transport and caveolin export from the Golgi complex.Traffic (Copenhagen, Denmark) · 2007Article
- EGF stimulates annexin 1-dependent inward vesiculation in a multivesicular endosome subpopulation.The EMBO journal · 2006Article
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- The annexin 2/S100A10 complex controls the distribution of transferrin receptor-containing recycling endosomes.Molecular biology of the cell · 2003Article
- Neutrophil interaction with inflamed postcapillary venule endothelium alters annexin 1 expression.The American journal of pathology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Annexin I is a member of a multigene family of Ca2+/phospholipid-binding proteins and a major substrate for the epidermal growth factor (EGF) receptor kinase, which has been implicated in membrane-related events along the endocytotic pathway, in particular in the sorting of internalized EGF receptors occurring in the multivesicular body. We analyzed in detail the intracellular distribution of this annexin by cell fractionation and immunoelectron microscopy. These studies used polyclonal as well as a set of species-specific monoclonal antibodies, whose epitopes were mapped to the lateral surface of the molecule next to a region thought to be involved in vesicle aggregation. Unexpectedly, the majority of annexin I was identified on early and not on multivesicular endosomes in a form that required micromolar levels of Ca2+ for the association. The specific cofractionation with early endosomes was also observed in transfected baby hamster kidney cells when the intracellular fate of ectopically expressed porcine annexin I was analyzed by using the species-specific monoclonal antibodies in Western blots of subcellular fractions. Interestingly, a truncation of the N-terminal 26, but not the N-terminal 13 residues of annexin I altered its intracellular distribution, shifting it from fractions containing early to those containing late and multivesicular endosomes. These findings underscore the regulatory importance of the N-terminal domain and provide evidence for an involvement of annexin I in early endocytotic processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.