ArticleJournal of muscle research and cell motility1996
A simple method for automatic tracking of actin filaments in the motility assay.
Article in Journal of muscle research and cell motility, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Characteristics of the right atrial and right ventricular contractility in a model of monocrotaline-induced pulmonary arterial hypertension.Journal of muscle research and cell motility · 2023Article
- Donor hearts in the Sydney Heart Bank: reliable control but is it 'normal' heart?Biophysical reviews · 2020Review
- Rapid time-stamped analysis of filament motility.Journal of muscle research and cell motility · 2018Article
- Abnormal contractility in human heart myofibrils from patients with dilated cardiomyopathy due to mutations in TTN and contractile protein genes.Scientific reports · 2017Article
- Investigations into the Sarcomeric Protein and CaFrontiers in physiology · 2017Article
- Myopathy-inducing mutation H40Y in ACTA1 hampers actin filament structure and function.Biochimica et biophysica acta · 2016Article
- Mutations in troponin T associated with Hypertrophic Cardiomyopathy increase Ca(2+)-sensitivity and suppress the modulation of Ca(2+)-sensitivity by troponin I phosphorylation.Archives of biochemistry and biophysics · 2016Article
- TPM3 deletions cause a hypercontractile congenital muscle stiffness phenotype.Annals of neurology · 2015Article
- Uncoupling of myofilament Ca2+ sensitivity from troponin I phosphorylation by mutations can be reversed by epigallocatechin-3-gallate.Cardiovascular research · 2015Article
- Mutations in repeating structural motifs of tropomyosin cause gain of function in skeletal muscle myopathy patients.Human molecular genetics · 2013Article
- Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.Biochimica et biophysica acta · 2013Article
- Antibodies covalently immobilized on actin filaments for fast myosin driven analyte transport.PloS one · 2012Article
- Molecular mechanism of the E99K mutation in cardiac actin (ACTC Gene) that causes apical hypertrophy in man and mouse.The Journal of biological chemistry · 2011Article
- Investigation of changes in skeletal muscle alpha-actin expression in normal and pathological human and mouse hearts.Journal of muscle research and cell motility · 2010Article
- Myosin motors drive long range alignment of actin filaments.The Journal of biological chemistry · 2010Article
- Random walks with thin filaments: application of in vitro motility assay to the study of actomyosin regulation.Journal of muscle research and cell motility · 2003Review
- Two mutations in troponin I that cause hypertrophic cardiomyopathy have contrasting effects on cardiac muscle contractility.The Biochemical journal · 2002Article
- A simple method for measuring the relative force exerted by myosin on actin filaments in the in vitro motility assay: evidence that tropomyosin and troponin increase force in single thin filaments.The Biochemical journal · 2000Article
- Motion determination in actin filament fluorescence images with a spatio-temporal orientation analysis method.Biophysical journal · 2000Article
- Troponin I and troponin T interact with troponin C to produce different Ca2+-dependent effects on actin-tropomyosin filament motility.The Biochemical journal · 1997Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A great deal of quantitative information about the actomyosin interaction can be obtained from the basic Kron and Spudich in vitro motility assay provided that care is taken to obtain consistency between experiments and that the data is examined comprehensively and not selectively. From observations of filament movement under a wide variety of conditions we have formulated the hypothesis that a large number of filaments moving over a short time period is indistinguishable from fewer filaments moving over a longer sequence of frames. This has been used to devise a simple automation of filament detection procedures. A sequence of images is digitized through a frame-grabber. If successive pairs of frames are compared the program will search for and detect the new position of every filament and show its vector on screen. Velocity is calculated and shown as a frequency histogram. The program regularly detects over 100 filaments moving in each pair of frames; usually a sequence of up to 15 pairs of frames are studied yielding 500-1000 vectors in total. The algorithm cannot deal with filaments that meet, cross or divide, however, when filaments are moving less than 2 microns between frames this is only a small proportion of the whole. The program outputs fraction of filaments motile, mean velocity with standard deviation and density of filaments (filaments microns-2). A cumulative frequency histogram gives an immediate visual indication of the performance of the population of filaments. Direct comparisons show that the data produced by automatic tracking is indistinguishable from manual tracking apart from the small apparent velocity of non-mobile filaments. The detection process takes about 5 min and requires little skill or judgement. This can lead to great increases in the rate of data analysis in motility work.
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