ArticlePlant physiology1996
Both 5' and 3' sequences of maize adh1 mRNA are required for enhanced translation under low-oxygen conditions.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Plant ADH1 promoter acts as an H3K27me3-associated hyper-long cold-responsive promoter.The Plant journal : for cell and molecular biology · 2025Article
- Plant translational reprogramming for stress resilience.Frontiers in plant science · 2023Review
- Arabidopsis thaliana alcohol dehydrogenase is differently affected by several redox modifications.PloS one · 2018Article
- 5'-non-transcribed flanking region and 5'-untranslated region play distinctive roles in sulfur deficiency induced expression ofPlant biotechnology (Tokyo, Japan) · 2017Article
- Maize reas1 Mutant Stimulates Ribosome Use Efficiency and Triggers Distinct Transcriptional and Translational Responses.Plant physiology · 2016Article
- Selective mRNA sequestration by OLIGOURIDYLATE-BINDING PROTEIN 1 contributes to translational control during hypoxia in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Using the candidate gene approach for detecting genes underlying seed oil concentration and yield in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
- Nuclear retention of the transcription factor NLP7 orchestrates the early response to nitrate in plants.Nature communications · 2013Article
- Evolution and expression of tandem duplicated maize flavonol synthase genes.Frontiers in plant science · 2012Article
- Post-transcriptional regulation of gene expression in plants during abiotic stress.International journal of molecular sciences · 2009Review
- The low-oxygen-induced NAC domain transcription factor ANAC102 affects viability of Arabidopsis seeds following low-oxygen treatment.Plant physiology · 2009Article
- Article
- Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation.Annals of botany · 2005Article
- Differential expression of genes encoding 1-aminocyclopropane-1-carboxylate synthase in Arabidopsis during hypoxia.Plant molecular biology · 2005Article
- LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis.Plant physiology · 2004Article
- DNA sequence-based "bar codes" for tracking the origins of expressed sequence tags from a maize cDNA library constructed using multiple mRNA sources.Plant physiology · 2003Article
- Enhancing the anaerobic response.Annals of botany · 2003Review
- Gene and enhancer trap transposable elements reveal oxygen deprivation-regulated genes and their complex patterns of expression in Arabidopsis.Annals of botany · 2003Article
- Molecular and cellular adaptations of maize to flooding stress.Annals of botany · 2003Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alcohol dehydrogenase-1 (ADH1) synthesis in O2-deprived roots of maize (Zea mays L.) results from induced transcription and selective translation of ADH1 mRNA. The effect of ADH1 mRNA sequences on message stability and translation was studied in protoplasts of the maize cell line P3377.5' capped and 3' polyadenylated mRNA constructs containing the firefly gene (luc) for luciferase (LUC) or the Escherichia coli gene (uidA) for beta-glucuronidase (GUS) coding region were synthesized in vitro and electroporated into protoplasts that were cultured at 40 or 5% O2. A LUC mRNA with a 17-nucleotide polylinker 5' untranslated region (UTR) was expressed 10-fold higher under aerobic conditions than under hypoxic conditions. Expression of five chimeric ADH1-GUS mRNAs was measured relative to this LUC mRNA. An mRNA containing the 5'-UTR and the first 18 codons of adh1 in a translational fusion with the GUS coding region and followed by the 3'-UTR of adh1 was expressed 57-fold higher at 5% O2. Progressive deletion of adh1 5'-UTR and coding sequences reduced expression of the GUS-mRNA at 5% O2, but had little impact on expression of 40% O2. Enhancement of expression in hypoxic protoplasts conferred by the adh1 5'-UTR and the first 26 codons decreased more than 3-fold when the adh1 3'-UTR was removed. In addition, the adh1 3'-UTR slightly inhibited expression in aerobic protoplasts. The physical half-lives of the GUS and LUC mRNAs were similar under both anaerobic and hypoxic conditions, indicating that expression levels were largely independent of mRNA stability. Thus, both adh1 5' and 3' mRNA sequences are required for enhanced translation in protoplasts under O2 deprivation.
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