ArticleMolecular & general genetics : MGG1996
Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stress.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73 citing papers in PubMed, 211 citations in OpenAlex.
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- Control of the rhizobium-legume symbiosis by the plant nitrogen demand is tightly integrated at the whole plant level and requires inter-organ systemic signaling.Frontiers in plant science · 2023Review
- A Chloroplast-Localized Glucose-6-Phosphate Dehydrogenase Positively Regulates Stripe Rust Resistance in Wheat.International journal of molecular sciences · 2022Article
- The Periplasmic Oxidoreductase DsbA Is Required for Virulence of the PhytopathogenInternational journal of molecular sciences · 2022Article
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath.Applied and environmental microbiology · 2021Article
- The Negative Effect of Protein Phosphatase Z1 Deletion on the Oxidative Stress Tolerance ofJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- The pentose phosphate pathway in industrially relevant fungi: crucial insights for bioprocessing.Applied microbiology and biotechnology · 2021Review
- Identification, Characterization, and Stress Responsiveness of Glucose-6-phosphate Dehydrogenase Genes in Highland Barley.Plants (Basel, Switzerland) · 2020Article
- Deciphering the crucial roles of AraC-type transcriptional regulator Cgl2680 on NADPH metabolism and L-lysine production in Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2020Article
- Transcriptome Profile of Yeast Strain Used for Biological Wine Aging Revealed Dynamic Changes of Gene Expression in Course of Flor Development.Frontiers in microbiology · 2020Article
- Carbohydrate, glutathione, and polyamine metabolism are central to Aspergillus flavus oxidative stress responses over time.BMC microbiology · 2019Article
- Functional definition of NrtR, a remnant regulator of NADFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Swift metabolite changes and leaf shedding are milestones in the acclimation process of grapevine under prolonged water stress.BMC plant biology · 2019Article
- Regulation of the Central Carbon Metabolism in Apple Fruit Exposed to Postharvest Low-Oxygen Stress.Frontiers in plant science · 2019Review
- Dropping in on lipid droplets: insights into cellular stress and cancer.Bioscience reports · 2018Review
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- Monitoring yeast mitochondria with peroxiredoxin-based redox probes: the influence of oxygen and glucose availability.Interface focus · 2017Article
13 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We have isolated several mutants of Saccharomyces cerevisiae that are sensitive to oxidative stress in a screen for elevated sensitivity to hydrogen peroxide. Two of the sixteen complementation groups obtained correspond to structural genes encoding enzymes of the pentose phosphate pathway. Allelism of the pos10 mutation (POS for peroxide sensitivity) to the zwf1/met1 mutants in the structural gene for glucose 6-phosphate dehydrogenase was reported previously. The second mutation, pos18, was complemented by transformation with a yeast genomic library. The open reading frame of the isolated gene encodes 238 amino acids. No detectable ribulose 5-phosphate epimerase activity was found in the pos18 mutant, suggesting that the corresponding structural gene is affected in this mutant. For that reason the gene was renamed RPE1 (for ribulose 5-phosphate epimerase). RPE1 was localized to chromosome X. The predicted protein has a molecular mass of 25966 Daltons, a codon adaptation index (CAI) of 0.32, and an isoelectric point of 5.82. Database searches revealed 32 to 37% identity with ribulose 5-phosphate epimerases of Escherichia coli, Rhodospirillum rubrum, Alcaligenes eutrophus and Solanum tuberosum. We have characterized RPE1 by testing enzyme activities in rpe1 deletion mutants and in strains that overexpress RPE1, and compared the hydrogen peroxide sensitivity of rpe1 mutants to that of other mutants in the pentose phosphate pathway. Interestingly, all mutants tested (glucose 6-phosphate dehydrogenase, gluconate 6-phosphate dehydrogenase, ribulose 5-phosphate epimerase, transketolase, transaldolase) are sensitive to hydrogen peroxide.
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