ArticleProceedings of the National Academy of Sciences of the United States of America1996
A cytoplasmic male sterility-associated mitochondrial protein causes pollen disruption in transgenic tobacco.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- A Systematic Review and Developmental Perspective on Origin of CMS Genes in Crops.International journal of molecular sciences · 2024Pooled it
- Review
- Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs.Plant biotechnology journal · 2024Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Did doubly uniparental inheritance (DUI) of mtDNA originate as a cytoplasmic male sterility (CMS) system?BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Advances in understanding the molecular mechanisms of cytoplasmic male sterility and restoration in rice.Plant reproduction · 2017Review
- Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.Plant reproduction · 2016Review
- MutS HOMOLOG1 silencing mediates ORF220 substoichiometric shifting and causes male sterility in Brassica juncea.Journal of experimental botany · 2016Article
- Article
- Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers.Frontiers in plant science · 2015Article
- Instability in mitochondrial membranes in Polima cytoplasmic male sterility of Brassica rapa ssp. chinensis.Functional & integrative genomics · 2014Article
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice.Nature genetics · 2013Article
- A male sterility-associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development.Journal of experimental botany · 2012Article
- A complete sequence and transcriptomic analyses of date palm (Phoenix dactylifera L.) mitochondrial genome.PloS one · 2012Article
- Intron hairpin and transitive RNAi mediated silencing of orfH522 transcripts restores male fertility in transgenic male sterile tobacco plants expressing orfH522.Plant molecular biology · 2011Article
- Substoichiometrically different mitotypes coexist in mitochondrial genomes of Brassica napus L.PloS one · 2011Article
- Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea.BMC plant biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen development is a consequence of mitochondrial dysfunction. To provide definitive evidence that expression of an abnormal mitochondrial gene can interrupt pollen development, a CMS-associated mitochondrial DNA sequence from common bean, orf239, was introduced into the tobacco nuclear genome. Several transformants containing the orf239 gene constructs, with or without a mitochondrial targeting sequence, exhibited a semi sterile or male-sterile phenotype. Expression of the gene fusions in transformed anthers was confirmed using RNA gel blotting, ELISA, and light and electron microscopic immunocytochemistry. Immunocytological analysis showed that the ORF239 protein could associate with the cell wall of aberrant developing microspores. This pattern of extracellular localization was earlier observed in the CMS common bean line containing orf239 in the mitochondrial genome. Results presented here demonstrate that ORF239 causes pollen disruption in transgenic tobacco plants and may do so without targeting of the protein to the mitochondrion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.