ArticleProceedings of the National Academy of Sciences of the United States of America1996
In vitro characterization of mutant yeast RNA polymerase II with reduced binding for elongation factor TFIIS.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Evolutionary conservation of the fidelity of transcription.Nature communications · 2023Article
- A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.PLoS genetics · 2014Article
- Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.The Journal of biological chemistry · 2013Article
- Dissection of Pol II trigger loop function and Pol II activity-dependent control of start site selection in vivo.PLoS genetics · 2012Article
- Overexpression of SNG1 causes 6-azauracil resistance in Saccharomyces cerevisiae.Current genetics · 2010Article
- Evidence that transcript cleavage is essential for RNA polymerase II transcription and cell viability.Molecular cell · 2010Article
- Knockdown of TFIIS by RNA silencing inhibits cancer cell proliferation and induces apoptosis.BMC cancer · 2008Article
- Mutations in the Saccharomyces cerevisiae RPB1 gene conferring hypersensitivity to 6-azauracil.Genetics · 2006Article
- Structural perspective on mutations affecting the function of multisubunit RNA polymerases.Microbiology and molecular biology reviews : MMBR · 2006Review
- A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- mRNA capping enzyme activity is coupled to an early transcription elongation.Molecular and cellular biology · 2004Article
- Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition.Genetics · 2004Article
- Article
- The Ras/PKA signaling pathway may control RNA polymerase II elongation via the Spt4p/Spt5p complex in Saccharomyces cerevisiae.Genetics · 2003Article
- Subnuclear localization and Cajal body targeting of transcription elongation factor TFIIS in amphibian oocytes.Molecular biology of the cell · 2003Article
- Use of RNA yeast polymerase II mutants in studying transcription elongation.Methods in enzymology · 2003Article
- Regulation of an IMP dehydrogenase gene and its overexpression in drug-sensitive transcription elongation mutants of yeast.The Journal of biological chemistry · 2001Article
- Saccharomyces cerevisiae transcription elongation mutants are defective in PUR5 induction in response to nucleotide depletion.Molecular and cellular biology · 2000Article
- Transcription elongation factor SII.BioEssays : news and reviews in molecular, cellular and developmental biology · 2000Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have reported previously the isolation and genetic characterization of mutations in the gene encoding the largest subunit of yeast RNA polymerase II (RNAPII), which lead to 6-azauracil (6AU)-sensitive growth. It was suggested that these mutations affect the functional interaction between RNAPII and transcription-elongation factor TFIIS because the 6AU-sensitive phenotype of the mutant strains was similar to that of a strain defective in the production of TFIIS and can be suppressed by increasing the dosage of the yeast TFIIS-encoding gene, PPR2, RNAPIIs were purified and characterized from two independent 6AU-sensitive yeast mutants and from wild-type (wt) cells. In vitro, in the absence of TFIIS, the purified wt polymerase and the two mutant polymerases showed similar specific activity in polymerization, readthrough at intrinsic transcriptional arrest sites and nascent RNA cleavage. In contrast to the wt polymerase, both mutant polymerases were not stimulated by the addition of a 3-fold molar excess of TFIIS in assays of promoter-independent transcription, readthrough or cleavage. However, stimulation of the ability of the mutant RNAPIIs to cleave nascent RNA and to read through intrinsic arrest sites was observed at TFIIS:RNAPII molar ratios greater than 600:1. Consistent with these findings, the binding affinity of the mutant polymerases for TFIIS was found to be reduced by more than 50-fold compared with that of the wt enzyme. These studies demonstrate that TFIIS has an important role in the regulation of transcription by yeast RNAPII and identify a possible binding site for TFIIS on RNAPII.
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