ReviewProceedings of the National Academy of Sciences of the United States of America1996
On the failure of de novo-designed peptides as biocatalysts.
Review in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Controlling complexity and water penetration in functional de novo protein design.Biochemical Society transactions · 2008Article
- Selection of peptides interfering with a ribosomal frameshift in the human immunodeficiency virus type 1.RNA (New York, N.Y.) · 2008Article
- Rational design of a novel calcium-binding site adjacent to the ligand-binding site on CD2 increases its CD48 affinity.Protein science : a publication of the Protein Society · 2008Article
- Biomimetic catalysis of diketopiperazine and dipeptide syntheses.Angewandte Chemie (International ed. in English) · 2008Article
- Functional and mechanistic analyses of biomimetic aminoacyl transfer reactions in de novo designed coiled coil peptides via rational active site engineering.Journal of the American Chemical Society · 2007Article
- Synthesis and selection of de novo proteins that bind and impede cellular functions of an essential mycobacterial protein.Applied and environmental microbiology · 2007Article
- Enzyme-like proteins by computational design.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Rational design of nascent metalloenzymes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Isolation of peptide aptamers that inhibit intracellular processes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Rational design of a scytalone dehydratase-like enzyme using a structurally homologous protein scaffold.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the elegance and efficiency of enzymatic catalysis have long tempted chemists and biochemists with reductionist leanings to try to mimic the functions of natural enzymes in much smaller peptides, such efforts have only rarely produced catalysts with biologically interesting properties. However, the advent of genetic engineering and hybridoma technology and the discovery of catalytic RNA have led to new and very promising alternative means of biocatalyst development. Synthetic chemists have also had some success in creating nonpeptide catalysts with certain enzyme-like characteristics, although their rates and specificities are generally much poorer than those exhibited by the best novel biocatalysts based on natural structures. A comparison of the various approaches from theoretical and practical viewpoints is presented. It is suggested that, given our current level of understanding, the most fruitful methods may incorporate both iterative selection strategies and rationally chosen small perturbations, superimposed on frameworks designed by nature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.