ArticleBiophysical journal1996
Lateral diffusion in planar lipid bilayers: a fluorescence recovery after photobleaching investigation of its modulation by lipid composition, cholesterol, or alamethicin content and divalent cations.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 126 citations in OpenAlex.
- Tutorial: biomembranes in hybrid living bioelectronics.Nature protocols · 2026Review
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
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- Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Annexin-V stabilizes membrane defects by inducing lipid phase transition.Nature communications · 2020Article
- Article
- Effects of lipid composition on membrane distribution and permeability of natural quinones.RSC advances · 2019Article
- Active Transport of Membrane Components by Self-Organization of the Min Proteins.Biophysical journal · 2019Article
- A new model for lipid monolayer and bilayers based on thermodynamics of irreversible processes.PloS one · 2019Article
- Increasing dietary oat fibre decreases the permeability of intestinal mucus.Journal of functional foods · 2016Article
- Omega-3 fatty acids, lipid rafts, and T cell signaling.European journal of pharmacology · 2016Review
- Lipid-Bilayer Dynamics Probed by a Carbon Dot-Phospholipid Conjugate.Biophysical journal · 2016Article
- Sodium alginate decreases the permeability of intestinal mucus.Food hydrocolloids · 2016Article
- Insertion and self-diffusion of a monotopic protein, the Aquifex aeolicus sulfide quinone reductase, in supported lipid bilayers.The European physical journal. E, Soft matter · 2015Article
- Beyond Saffman-Delbruck approximation: a new regime for 2D diffusion of α-hemolysin complexes in supported lipid bilayer.The European physical journal. E, Soft matter · 2012Article
- Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues.Plant methods · 2012Article
- Exploring the membrane mechanism of the bioactive peptaibol ampullosporin a using lipid monolayers and supported biomimetic membranes.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2010Article
- Polymer-supported lipid shells, onions, and flowers.Soft matter · 2008Article
- Intermonolayer friction and surface shear viscosity of lipid bilayer membranes.Biophysical journal · 2007Article
- Influence of lipid chemistry on membrane fluidity: tail and headgroup interactions.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In spite of the fact that planar lipid bilayers are still the best-suited artificial membrane system for the study of reconstituted ion channels and receptors, data dealing with their physical characterization, especially as regards dynamics, are scanty. A combined electrical and optical chamber was designed and allowed fluorescence recovery after photobleaching recovery curves to be recorded from stable virtually solvent-free bilayers. D, the lateral diffusion coefficient of N-(7-nitrobenzoyl-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn- glycero-3-phosphoethanolamine, was found to be relatively insensitive to the phospholipid composition (headgroup, chain unsaturation, etc.), whereas inclusion of 33-50% cholesterol in the membrane reduced D by a factor of 2. Divalent cations significantly reduced D of negatively charged bilayers. These results compare well with data gathered on other model and natural systems. In addition, the incorporation of the voltage-dependent pore-former alamethicin did slightly reduce lipid lateral mobility. This study demonstrates the feasibility of such experiments with planar bilayers, which are amenable to physical constraints, and thus offers new opportunities for systematic studies of structure-function relationships in membrane-associating molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.