ArticleGlycoconjugate journal1996
Characterization of the binding specificity of Anguilla anguilla agglutinin (AAA) in comparison to Ulex europaeus agglutinin I (UEA-I).
Article in Glycoconjugate journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Coordination-cage-templated multivalent glycan arrays: effects of mixed-glycan composition and chain length on lectin binding.Chemical science · 2026Article
- Specificity of widely used lectins as probed with oligosaccharide and plant polysaccharide arrays.Histochemistry and cell biology · 2024Article
- A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities.ACS chemical biology · 2022Article
- Ulex Europaeus Agglutinin-I-Based Magnetic Isolation for the Efficient and Specific Capture of SW480 Circulating Colorectal Tumor Cells.ACS omega · 2022Article
- Enzymatic elaboration of oxime-linked glycoconjugates in solution and on liposomes.Journal of materials chemistry. B · 2022Article
- Identification of α1,2-fucosylated signaling and adhesion molecules in head and neck squamous cell carcinoma.Glycobiology · 2022Article
- Glycan array analysis of Pholiota squarrosa lectin and other fucose-oriented lectins.Glycobiology · 2021Article
- Predictive modeling of complex ABO glycan phenotypes by lectin microarrays.Blood advances · 2020Article
- Altered glycosylation of glycodelin in endometrial carcinoma.Laboratory investigation; a journal of technical methods and pathology · 2020Article
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- Application of Lectin Microarrays for Biomarker Discovery.ChemistryOpen · 2020Review
- An F-type lectin domain directs the activity of Streptosporangium roseum alpha-l-fucosidase.Glycobiology · 2018Article
- The Interaction of the Gut Microbiota with the Mucus Barrier in Health and Disease in Human.Microorganisms · 2018Review
- Uncovering the Binding Specificities of Lectins with Cells for Precision Colorectal Cancer Diagnosis Based on Multimodal Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Targeting cancer-specific glycans by cyclic peptide lectinomimics.Amino acids · 2017Article
- The Human Glycoprotein Salivary Agglutinin Inhibits the Interaction of DC-SIGN and Langerin with Oral Micro-Organisms.Journal of innate immunity · 2016Article
- Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- High-sensitivity analytical approaches for the structural characterization of glycoproteins.Chemical reviews · 2013Review
- Automated motif discovery from glycan array data.Omics : a journal of integrative biology · 2012Article
- Identification of fucosylated glycoconjugates in Xenopus laevis testis by lectin histochemistry.Histochemistry and cell biology · 2010Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using immunochemical and immunohistochemical methods, the binding site of Anguilla anguilla agglutinin (AAA) was characterized and compared with the related fucose-specific lectin from Ulex europaeus (UEA-I). In solid-phase enzyme-linked immunoassays, the two lectins recognized Fuc alpha 1-2Gal beta-HSA. AAA additionally cross-reacted with neoglycolipids bearing lacto-N-fucopentaose (LNFP) I [H type 1] and II [Le(a)] and lactodifucotetraose (LDFT) as glycan moieties. UEA-I, on the other hand, bound to a LDFT-derived neoglycolipid but not to the other neoglycolipids tested. Binding of AAA to gastric mucin was competitively neutralized by Le(a)-specific monoclonal antibodies. UEA-I binding, on the other hand, was reduced after co-incubation with H type 2- and Le(y)-specific monoclonal antibodies. According to our results, AAA reacts with fucosylated type 1 chain antigens, whereas UEA-I binds only to the alpha 1-2-fucosylated LDFT-derived neoglycolipid. In immunohistochemical studies, the reactivity of AAA and UEA-I in normal pyloric mucosa from individuals with known Lewis and secretor status was analysed. AAA showed a broad reaction in the superficial pyloric mucosa from secretors and non-secretors, but AAA reactivity was more pronounced in Le(a+b-) individuals. On the other hand, UEA-I stained the superficial pyloric mucosa only from secretor individuals. A staining of deep mucous glands by the lectins was found in all specimens. Both reacted with most human carcinomas of different origin. Slight differences in their binding pattern were observed and may be explained by the different fine-specificities of the lectins.
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