ArticleNucleic acids research1996
A small circular TAR RNA decoy specifically inhibits Tat-activated HIV-1 transcription.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 70 citations in OpenAlex.
- Circular RNA functions in HIV-1 infection.RNA biology · 2026Review
- The therapeutic potential of circular RNAs.Nature reviews. Genetics · 2025Review
- Review
- CircRNP complexes: from nature to design.Journal of molecular cell biology · 2023Article
- The design and synthesis of circular RNAs.Methods (San Diego, Calif.) · 2021Review
- The emerging role and significance of circular RNAs in viral infections and antiviral immune responses: possible implication as theranostic agents.RNA biology · 2021Review
- Evolution of a highly functional circular DNA aptamer in serum.Nucleic acids research · 2020Article
- Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand.Nucleic acids research · 2020Article
- Molecular entrapment by RNA: an emerging tool for disrupting protein-RNA interactionsRNA biology · 2020Article
- Differential expression profile study and gene function analysis of maternal foetal-derived circRNA for screening for Down's syndrome.Experimental and therapeutic medicine · 2020Article
- A 360° view of circular RNAs: From biogenesis to functions.Wiley interdisciplinary reviews. RNA · 2018Review
- Identification and characterization of circular RNAs in the silkworm midgut following Bombyx mori cytoplasmic polyhedrosis virus infection.RNA biology · 2018Article
- Early history of circular RNAs, children of splicing.RNA biology · 2017Article
- Roles of Circular RNAs in Neurologic Disease.Frontiers in molecular neuroscience · 2016Review
- RNA circularization strategies in vivo and in vitro.Nucleic acids research · 2015Review
- Zinc finger protein designed to target 2-long terminal repeat junctions interferes with human immunodeficiency virus integration.Human gene therapy · 2012Article
- Toward targeting RNA structure: branched peptides as cell-permeable ligands to TAR RNA.ACS chemical biology · 2012Article
- Self-circularizing 5'-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica.Scientific reports · 2012Article
- Oligomeric nucleic acids as antivirals.Molecules (Basel, Switzerland) · 2011Review
- Molecular recognition elements: DNA/RNA-aptamers to proteins.Biochemistry (Moscow) Supplement. Series B, Biomedical chemistry · 2010Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Linear TAR RNA has previously been used as a decoy to inhibit HIV-1 transcription in vitro and HIV-1 replication in vivo. A 48 nucleotide circular RNA containing the stem, bulge and loop of the HIV-1 TAR element was synthesized using the self-splicing activity of a group I permuted intron-exon and was tested for its ability to function as a TAR decoy in vitro. This small circular TAR molecule was exceptionally stable in HeLa nuclear extracts, whereas a similar linear TAR molecule was rapidly degraded. The TAR circle bound specifically to Tfr38, a peptide containing the TAR-binding region of Tat. The ability of Tat to trans-activate transcription from the HIV-1 promoter in vitro was efficiently inhibited by circular TAR RNA but not by TAR circles that contained either bulge or loop mutations. TAR circles did not inhibit transactivation exclusively by binding to Tat since this inhibition was not reversed by adding excess Tat to the transcription reaction. Together, these data suggest that TAR circles act as decoys that inhibit transactivation by binding to Tat and at least one cellular factor. These data also demonstrate the utility of small circular RNA molecules as tools for biochemical studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.