ArticleThe Biochemical journal1996
Structural requirements for annexin I-S100C complex-formation.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 89 citations in OpenAlex.
- Revisiting the role of Annexins in membrane trafficking.Cellular and molecular life sciences : CMLS · 2025Review
- FLIM-FRET-based analysis of S100A11/annexin interactions in living cells.FEBS open bio · 2024Article
- Zooming into the Dark Side of Human Annexin-S100 Complexes: Dynamic Alliance of Flexible Partners.International journal of molecular sciences · 2020Review
- Calgizzarin (S100A11): a novel inflammatory mediator associated with disease activity of rheumatoid arthritis.Arthritis research & therapy · 2017Article
- A multiplexed, targeted mass spectrometry assay of the S100 protein family uncovers the isoform-specific expression in thyroid tumours.BMC cancer · 2015Article
- Phosphorylation of annexin A1 by TRPM7 kinase: a switch regulating the induction of an α-helix.Biochemistry · 2011Article
- Crystal structure of metastasis-associated protein S100A4 in the active calcium-bound form.Journal of molecular biology · 2008Article
- Characterization of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migration.The Journal of biological chemistry · 2008Article
- Membrane-induced folding and structure of membrane-bound annexin A1 N-terminal peptides: implications for annexin-induced membrane aggregation.Biophysical journal · 2008Article
- Calcium-dependent and -independent interactions of the S100 protein family.The Biochemical journal · 2006Review
- Macrophage surface expression of annexins I and II in the phagocytosis of apoptotic lymphocytes.Molecular biology of the cell · 2004Article
- Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P.Molecular biology of the cell · 2003Article
- Promoting detachment of neutrophils adherent to murine postcapillary venules to control inflammation: effect of lipocortin 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S100C is a member of the S100 family of EF-hand-type Ca(2+)-binding proteins which are thought to bind to and thereby regulate the activity of cellular target proteins in a Ca(2+)-dependent manner. An intracellular ligand for S100C is the Ca2+/phospholipid-binding protein annexin I and we show here that complex-formation is mediated through unique domains within S100C and annexin I. Using a proteolytically truncated annexin I derivative as well as a number of N-terminal annexin I peptides in liposome co-pelleting and ligand-blotting assays we map the S100C-binding site to the N-terminal 13 residues of annexin I. Similar analyses employing recombinantly expressed S100C mutants reveal that residues D91 to 194 in the unique C-terminal extension of this S100 protein are indispensable for annexin I binding. Interaction between S100C and an N-terminal annexin I peptide containing a tryptoplan at position 11 can also be monitored by fluorescence emission spectroscopy after tryptophan excitation. This analysis indicates that the local environment of the tryptophan in annexin I becomes less aqueous on S100C binding, suggesting a hydrophobic nature of the protein-protein interaction. Thus the structural basis of the annexin 1-S100C complex-formation probably resembles to a large extent that of the well-characterized annexin II-p11 interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.