ArticleMolecular biology of the cell1996
Purification and identification of FOAD-II, a cytosolic protein that regulates secretion in streptolysin-O permeabilized mast cells, as a rac/rhoGDI complex.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 58 citations in OpenAlex.
- Identification of secernin 1 as a novel immunotherapy target for gastric cancer using the expression profiles of cDNA microarray.Cancer science · 2006Article
- RhoGDI: multiple functions in the regulation of Rho family GTPase activities.The Biochemical journal · 2005Review
- Facilitation of Ca(2+)-dependent exocytosis by Rac1-GTPase in bovine chromaffin cells.The Journal of physiology · 2003Article
- Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells.Molecular biology of the cell · 2002Article
- Phosphatidylinositol transfer proteins and protein kinase C make separate but non-interacting contributions to the phosphorylation state necessary for secretory competence in rat mast cells.The Biochemical journal · 2001Article
- Activation of exocytosis by cross-linking of the IgE receptor is dependent on ADP-ribosylation factor 1-regulated phospholipase D in RBL-2H3 mast cells: evidence that the mechanism of activation is via regulation of phosphatidylinositol 4,5-bisphosphate synthesis.The Biochemical journal · 2000Article
- Cdc42 and Rac stimulate exocytosis of secretory granules by activating the IP(3)/calcium pathway in RBL-2H3 mast cells.The Journal of cell biology · 2000Article
- Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.Molecular biology of the cell · 2000Article
- Regulation of exocytosis from rat peritoneal mast cells by G protein beta gamma-subunits.The EMBO journal · 1998Article
- Induction of exocytosis from permeabilized mast cells by the guanosine triphosphatases Rac and Cdc42.Molecular biology of the cell · 1998Article
- Characterization of a Rac1- and RhoGDI-associated lipid kinase signaling complex.Molecular and cellular biology · 1998Article
- Regulation of cell-cell adhesion by rac and rho small G proteins in MDCK cells.The Journal of cell biology · 1997Article
- Rho guanine nucleotide dissociation inhibitor protein (RhoGDI) inhibits exocytosis in mast cells.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mast cells permeabilized by treatment with streptolysin-O in the presence of Ca2+ and GTP-gamma-S can secrete almost 100% of their contained N-acetyl-beta-D-glucosaminidase. If these stimuli are provided to the permeabilized cells after a delay, the response is diminished and the ability of the cells to undergo secretion runs down progressively over a period of about 30 min. This is thought to be due to the loss of key proteins involved in the exocytotic mechanism. Using this effect as the basis of a biological assay, we have isolated a protein from bovine brain cytosol that retards the loss of responsiveness to stimulation by Ca2+ and GTP-gamma-S. Purification of this protein and peptide sequencing have enabled us to identify it as the small GTP-binding protein rac complexed to the guanine nucleotide exchange inhibitor rhoGDI. Both proteins are required to retard the loss of the secretory response, while purified rhoGDI applied alone accelerates the rundown.
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