ArticleThe Journal of general physiology1996
Subconductance block of single mechanosensitive ion channels in skeletal muscle fibers by aminoglycoside antibiotics.
Article in The Journal of general physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- Synergistic Cellular Responses Conferred by Concurrent Optical and Magnetic Stimulation Are Attenuated by Simultaneous Exposure to Streptomycin: An Antibiotic Dilemma.Bioengineering (Basel, Switzerland) · 2024Article
- Mechanotransduction for Muscle Protein Synthesis via Mechanically Activated Ion Channels.Life (Basel, Switzerland) · 2023Review
- Ambient and supplemental magnetic fields promote myogenesisFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Piezo channels and GsMTx4: Two milestones in our understanding of excitatory mechanosensitive channels and their role in pathology.Progress in biophysics and molecular biology · 2017Review
- Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields.Scientific reports · 2017Article
- Arrhythmogenic effects by local left ventricular stretch: effects of flecainide and streptomycin.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Gentamicin blocks the ACh-induced BK current in guinea pig type II vestibular hair cells by competing with Ca²⁺ at the L-type calcium channel.International journal of molecular sciences · 2014Article
- Aminoglycoside block of P2X2 receptors heterologously expressed in Xenopus laevis oocytes.Purinergic signalling · 2010Article
- Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.Plant cell reports · 2010Review
- [Cellular regulation of anabolism and catabolism in skeletal muscle during immobilisation, aging and critical illness].Wiener klinische Wochenschrift · 2007Review
- On the interaction of neomycin with the slow vacuolar channel of Arabidopsis thaliana.The Journal of general physiology · 2006Article
- Mechanisms of stretch-induced muscle damage in normal and dystrophic muscle: role of ionic changes.The Journal of physiology · 2005Review
- Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouse.The Journal of physiology · 2005Article
- Voltage-dependent inhibition of rat skeletal muscle sodium channels by aminoglycoside antibiotics.Pflugers Archiv : European journal of physiology · 2004Article
- Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials.Biophysical journal · 2002Article
- Gentamicin blocks ACh-evoked K+ current in guinea-pig outer hair cells by impairing Ca2+ entry at the cholinergic receptor.The Journal of physiology · 2000Article
- Streptomycin inhibition of myogenic tone, K+-induced force and block of L-type calcium current in rat cerebral arteries.The Journal of physiology · 1998Article
- Evidence TRPV4 contributes to mechanosensitive ion channels in mouse skeletal muscle fibers.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activity of single mechanosensitive channels was recorded from cell-attached patches on acutely isolated skeletal muscle fibers from the mouse. The experiments were designed to investigate the mechanism of channel block produced by externally applied aminoglycoside antibiotics. Neomycin and other aminoglycosides reduced the amplitude of the single-channel current at negative membrane potentials. The block was concentration-dependent, with a half-maximal concentration of approximately 200 microM. At high drug concentrations, however, block was incomplete with roughly one third of the current remaining unblocked. Neomycin also caused the channel to fluctuate between the open state and a subconductance level that was also roughly one third the amplitude of the fully open level. An analysis of the kinetics of the subconductance fluctuations was consistent with a bimolecular reaction between an aminoglycoside molecule and the open channel (kon = approximately 1 x 10(6) M-1s-1 and koff = approximately 400 s-1 at -60 mV). Increasing the external pH reduced both the rapid block of the open channel and the frequency of the subconductance fluctuations, as if both blocking actions were produced by a single active drug species with a pKa = approximately 7.5. The results are interpreted in terms of a mechanism in which an aminoglycoside molecule partially occludes ion flow through the channel pore.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.