ArticleThe Journal of general physiology1996
Interaction of internal Ba2+ with a cloned Ca(2+)-dependent K+ (hslo) channel from smooth muscle.
Article in The Journal of general physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 48 citations in OpenAlex.
- Mutation in pore-helix modulates interplay between filter gate and Ba2+ block in a Kcv channel pore.The Journal of general physiology · 2024Article
- Mg²⁺ modulation of the single-channel properties of KCa3.1 in human erythroleukemia cells.Pflugers Archiv : European journal of physiology · 2014Article
- The first transmembrane domain (TM1) of β2-subunit binds to the transmembrane domain S1 of α-subunit in BK potassium channels.FEBS letters · 2012Article
- Coupling and cooperativity in voltage activation of a limited-state BK channel gating in saturating Ca2+.The Journal of general physiology · 2010Article
- Alpha5beta1 integrin engagement increases large conductance, Ca2+-activated K+ channel current and Ca2+ sensitivity through c-src-mediated channel phosphorylation.The Journal of biological chemistry · 2010Article
- An extracellular Cu2+ binding site in the voltage sensor of BK and Shaker potassium channels.The Journal of general physiology · 2008Article
- Intrinsic electrostatic potential in the BK channel pore: role in determining single channel conductance and block.The Journal of general physiology · 2008Article
- Mg2+ enhances voltage sensor/gate coupling in BK channels.The Journal of general physiology · 2008Article
- State-dependent block of BK channels by synthesized shaker ball peptides.The Journal of general physiology · 2006Article
- Role of charged residues in the S1-S4 voltage sensor of BK channels.The Journal of general physiology · 2006Article
- Generation of functional fluorescent BK channels by random insertion of GFP variants.The Journal of general physiology · 2005Article
- Cysteine modification alters voltage- and Ca(2+)-dependent gating of large conductance (BK) potassium channels.The Journal of general physiology · 2005Article
- Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part II: Neutralization of aspartate 292 reduces long channel openings and gating current slow component.The Journal of general physiology · 2004Article
- Unique inner pore properties of BK channels revealed by quaternary ammonium block.The Journal of general physiology · 2004Article
- Mapping the BKCa channel's "Ca2+ bowl": side-chains essential for Ca2+ sensing.The Journal of general physiology · 2004Article
- Protons block BK channels by competitive inhibition with K+ and contribute to the limits of unitary currents at high voltages.The Journal of general physiology · 2004Article
- A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectification.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Coupling between voltage sensor activation, Ca2+ binding and channel opening in large conductance (BK) potassium channels.The Journal of general physiology · 2002Article
- Elimination of the BK(Ca) channel's high-affinity Ca(2+) sensitivity.The Journal of general physiology · 2002Article
- Specific localization of an inwardly rectifying K(+) channel, Kir4.1, at the apical membrane of rat gastric parietal cells; its possible involvement in K(+) recycling for the H(+)-K(+)-pump.The Journal of physiology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have studied potassium currents through a cloned Ca(2+)-dependent K+ channel (hslo) from human myometrium. Currents were recorded in inside-out macropatches from membranes of Xenopus laevis oocytes. In particular, the inactivation-like process that these channels show at high positive potentials was assessed in order to explore its molecular nature. This current inhibition conferred a bell shape to the current-voltage curves. The kinetic and voltage dependence of this process suggested the possibility of a Ba2+ block. There were the following similarities between the inactivation process observed at zero-added Ba2+ and the internal Ba2+ block of hslo channels: (a) in the steady state, the voltage dependence of the current inhibition observed at zero-added Ba2+ was the same as the voltage dependence of the Ba2+ block; (b) the time constant for recovery from current decay at zero-added Ba2+ was the same as the time constant for current recovery from Ba2+ blockade; and (c) current decay was largely suppressed in both cases by adding a Ba2+ chelator [(+)-18-crown-6-tetracarboxylic acid] to the internal solution. In our experimental conditions, we determined that the Kd for the complex chelator-Ba2+ is 1.6 x 10(-10) M. We conclude that the current decay observed at zero-added Ba2+ to the internal solution is due to contaminant Ba2+ present in our solutions (approximately 70 nM) and not to an intrinsic gating process. The Ba2+ blocking reaction in hslo channels is bimolecular. Ba2+ binds to a site (Kd = 0.36 +/- 0.05 mM at zero applied voltage) that senses 92 +/- 25% of the potential drop from the internal membrane surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.