ArticleThe Journal of physiology1996
Inwardly rectifying K+ channels in freshly dissociated coronary endothelial cells from guinea-pig heart.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- Boosting the signal: Endothelial inward rectifier KMicrocirculation (New York, N.Y. : 1994) · 2017Review
- Inward rectifier potassium (Kir2.1) channels as end-stage boosters of endothelium-dependent vasodilators.The Journal of physiology · 2016Article
- KATP Channels in the Cardiovascular System.Physiological reviews · 2016Review
- Inward Rectifier K+ Currents Are Regulated by CaMKII in Endothelial Cells of Primarily Cultured Bovine Pulmonary Arteries.PloS one · 2015Article
- Membrane hyperpolarization during human sperm capacitation.Molecular human reproduction · 2014Article
- No independent, but an interactive, role of calcium-activated potassium channels in human cutaneous active vasodilation.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Multiplicity of effectors of the cardioprotective agent, diazoxide.Pharmacology & therapeutics · 2013Review
- Physiological role of inward rectifier K(+) channels in vascular smooth muscle cells.Pflugers Archiv : European journal of physiology · 2008Review
- Ca2+-activated K+ channels in murine endothelial cells: block by intracellular calcium and magnesium.The Journal of general physiology · 2008Article
- Molecular basis of inward rectification: polyamine interaction sites located by combined channel and ligand mutagenesis.The Journal of general physiology · 2004Article
- Freshly isolated bovine coronary endothelial cells do not express the BK Ca channel gene.The Journal of physiology · 2002Article
- Multigene family isoform profiling from blood cell lineages.BMC genomics · 2002Article
- Blockade by 18beta-glycyrrhetinic acid of intercellular electrical coupling in guinea-pig arterioles.The Journal of physiology · 1998Article
- Membrane currents and the resting membrane potential in cultured bovine pulmonary artery endothelial cells.The Journal of physiology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Inwardly rectifying K+ (IK(IR)) currents of freshly dissociated coronary endothelial cells from guinea-pig heart were investigated with the perforated-patch technique. 2. The whole-cell current-voltage relationship of endothelial cells showed strong inward rectification. Increasing the extracellular K+ resulted in an increase of inward currents. The slope conductance of the cells in the potential range negative to the calculated potassium equilibrium potential (EK) with 5, 60 and 150 mM external potassium was 0.18 +/- 0.14, 0.55 +/- 0.50 and 0.63 +/- 0.29 nS (mean +/- S.D.), respectively. 3. To quantify the steepness of inward rectification, the voltage dependence of the chord conductance of the cells was fitted with a Boltzmann function. The slope factor k describing the steepness of the relationship was 6.8 +/- 1.5 mV. 4. Extracellular barium induced a potential- and time-dependent block of inward currents through endothelial KIR channels. Half-maximum inhibition of IK(IR) currents was achieved with < or = 1 microM barium at a membrane potential of -70 mV in a solution containing 60 mM K+. 5. Whole-cell inward currents revealed the opening and closing of single KIR channels. The single-channel conductance was 26 +/- 3 pS with 60 mM external K+ and 33 +/- 6 pS with 150 mM external K+. 6. Our results suggest that the electrical properties of freshly dissociated endothelial cells are to a large extent determined by five to sixty active strong inwardly rectifying K+ (KIR) channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.