ArticleThe Journal of physiology1996
Monitoring calcium in turtle hair cells with a calcium-activated potassium channel.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 74 citations in OpenAlex.
- Assessing evidence for adaptive evolution in two hearing-related genes important for high-frequency hearing in echolocating mammals.G3 (Bethesda, Md.) · 2021Article
- SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials.Frontiers in cellular neuroscience · 2021Article
- Synaptic cleft microenvironment influences potassium permeation and synaptic transmission in hair cells surrounded by calyx afferents in the turtle.The Journal of physiology · 2020Article
- EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Synaptic calcium regulation in hair cells of the chicken basilar papilla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A 'calcium capacitor' shapes cholinergic inhibition of cochlear hair cells.The Journal of physiology · 2014Review
- Ultrastructure of cisternal synapses on outer hair cells of the mouse cochlea.The Journal of comparative neurology · 2014Article
- Response properties from turtle auditory hair cell afferent fibers suggest spike generation is driven by synchronized release both between and within synapses.Journal of neurophysiology · 2013Article
- Permeation properties of the hair cell mechanotransducer channel provide insight into its molecular structure.Journal of neurophysiology · 2012Article
- Onset of cholinergic efferent synaptic function in sensory hair cells of the rat cochlea.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Article
- Calcium-dependent control of temporal processing in an auditory interneuron: a computational analysis.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2010Article
- Monitoring transient Ca2+ dynamics with large-conductance Ca2+-dependent K+ channels at active zones in frog saccular hair cells.Neuroscience · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- Expression of the SK2 calcium-activated potassium channel is required for cholinergic function in mouse cochlear hair cells.The Journal of physiology · 2008Article
- Calcium-dependent inactivation of calcium channels in cochlear hair cells of the chicken.The Journal of physiology · 2007Article
- Genetic deletion of SK2 channels in mouse inner hair cells prevents the developmental linearization in the Ca2+ dependence of exocytosis.The Journal of physiology · 2007Article
- Ca2+ current of frog vestibular hair cells is modulated by intracellular ATP but not by long-lasting depolarisation.European biophysics journal : EBJ · 2007Article
- Divergent calcium signaling in RBCs from Tropidurus torquatus (Squamata--Tropiduridae) strengthen classification in lizard evolution.BMC physiology · 2007Article
- Hair cell ribbon synapses.Cell and tissue research · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. An apamin-sensitive Ca(2+)-activated K+ channel was characterized in turtle hair cells and utilized to monitor submembranous intracellular Ca2+ and to evaluate the concentration of the mobile endogenous calcium buffer. 2. Isolated hair cells were voltage clamped with whole-cell patch electrodes filled with a Cs(+)-based intracellular solution to block the large-conductance Ca(2+)-activated K+ (BK) channel. Ca2+ currents evoked by depolarization were followed by inward tail currents lasting several hundred milliseconds. Both the Ca2+ current and slow tail current were abolished by nifedipine. 3. The tail current was carried by K+ and Cs+ (relative permeabilities PCa/PK = 0.22), and was fully blocked by 0.1 microM apamin and half blocked by 5 mM external TEA. These properties suggest the tail current flows through a Ca(2+)-activated K+ channel distinct from the BK channels. 4. Intracellular Ca2+ was imaged with a confocal microscope in hair cells filled with the indicator Calcium Green-5N introduced via the patch pipette. Increases in Ca2+ evoked by depolarization were localized to hotspots on the basolateral surface of the cell. The time course of the tail current closely matched the fast component of the fluorescenece monitored at a hotspot. 5. Ca(2+)-ATPase pump inhibitors thapsigargin, 2,4-di-(t-butyl)hydroquinone (BHQ) and vanadate, which are known to influence calcium regulation in turtle hair cells, prolonged the time course of the tail current, supporting the idea that the channel monitors cytoplasmic Ca2+. 6. The mobile endogenous buffer was estimated by combining perforated-patch and whole-cell recordings on a single cell. After recording tail currents with an amphotericin-perforated patch, the patch was ruptured to obtain the whole-cell mode, thus allowing washout of soluble cytoplasmic proteins and exchange with pipette buffers. By varying the concentration of Ca2+ buffer in the pipette, the mobile endogenous buffer was found to be equivalent to about 1 mM BAPTA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.