ArticleJournal of clinical microbiology1996
Use of multiple PCR primer sets for optimal detection of human papillomavirus.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- A Novel Method for Predicting Oncogenic Types of Human Papillomavirus.Diagnostics (Basel, Switzerland) · 2025Article
- The Methylation Test Is Highly Sensitive for HPV-Associated Endocervical Adenocarcinoma and Could Be Helpful as a Cytological Ancillary Test in Women With a PAP-Smear Diagnosis of Severe Glandular Lesion (AGC-NEO+).Cytopathology : official journal of the British Society for Clinical Cytology · 2025Article
- HPV-driven breast carcinogenesis: associations with tumor severity, Ki67 expression and metastasis.Infectious agents and cancer · 2025Article
- Robust HPV-16 Detection Workflow for Formalin-Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma.Cancer medicine · 2025Article
- Molecular Characterization of BovineVeterinary medicine international · 2025Article
- Comparison of multiplex PCR capillary electrophoresis assay and PCR-reverse dot blot assay for human papillomavirus DNA genotyping detection in cervical cancer tissue specimens.Frontiers in public health · 2024Article
- Head-to-Head Comparison of DH3 HPV Test and HC2 Assay for Detection of High-Risk HPV Infection in Residual Cytology Samples from Cervical Cancer Screening Setting: Baseline and 3-Year Longitudinal Data.Microbiology spectrum · 2022Article
- Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples.PloS one · 2021Article
- Rational genomic optimization of DNA detection for human papillomavirus type 16 in head and neck squamous cell carcinoma.Head & neck · 2020Article
- Establishment and Application of Multiplex PCR for Simultaneously DetectingFrontiers in veterinary science · 2020Article
- Article
- The potential of RNA as a target for national screening of pre-cancer.Journal of public health in Africa · 2018Article
- Profiling of serum antibodies against human papillomavirus antigens in Korean women with cervical intraepithelial neoplasia and cervical cancer.Cancer medicine · 2018Article
- CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method.Scientific reports · 2018Article
- Integration sites of human papillomavirus 18 in esophageal cancer samples.Oncology letters · 2018Article
- Prevalence of the integration status for human papillomavirus 16 in esophageal carcinoma samples.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2018Article
- Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells.Experimental and therapeutic medicine · 2017Article
- HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization.PloS one · 2017Article
- Integration of human papillomavirus 16 in esophageal carcinoma samples.Infectious agents and cancer · 2017Article
- How many papillomavirus species can go undetected in papilloma lesions?Scientific reports · 2016Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using multiple PCR primer sets, we tried to optimize the detection of human papillomavirus (HPV) in DNA samples isolated from 361 frozen biopsy specimens from patients with invasive cervical carcinomas. The HPVs detected were placed into three distinct groups, including group I/Inex at Telelab (Skien, Norway) and group Ineg and group II at the Norwegian Radium Hospital (Oslo, Norway). The consensus primer sets were Oli-1b-oli-2i, My09-My11, Gp5-Gp6, and Gp(5+)-Gp6+ from the HPV L1 gene and CpI-CpIIG from the E1 gene. Using these consensus primers together with the type-specific primers from E6-E7, we found that 355 patients (98%) were HPV positive. Type-specific primers for HPV types 11, 16, 18, 31, 33, and 35 detected more HPV-infected patients than the most sensitive consensus primer set, while the three consensus primer sets My, Gp/Gp+, and Cp together detected more HPV-positive patients than the type-specific primers. Testing of sensitivity of the PCR with SiHa cells serially diluted in lymphocytes (HPV-negative cells) indicated a detection limit of 6,300 HPV type 16 DNA copies with consensus primers (My, Gp+, and Cp) and 126 original HPV type 16 DNA copies with type-specific primers. Comparison of the amplification results for consensus L1 primers and type-specific E6-E7 primers indicated the presence of L1 deletions in 23 of 56 samples. The conclusion is that in PCR detection systems, multiple consensus primers and type-specific primers should be used in order to detect all patients harboring HPV.
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