ArticleMolecular biology of the cell1996
Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- From amoeboid myosin to unique targeted medicines for a genetic cardiac disease.Frontiers in physiology · 2024Review
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- Analysis of lncRNA in the skeletal muscle of rabbits at different developmental stages.Frontiers in veterinary science · 2022Article
- Single-molecule analysis reveals that regulatory light chains fine-tune skeletal myosin II function.The Journal of biological chemistry · 2020Article
- A novel missense mutation in the MYH7 gene causes an uncharacteristic phenotype of myosin storage myopathy: a case report.BMC medical genetics · 2019Article
- Article
- A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis.Scientific reports · 2017Article
- Developmental MYH3 Myopathy Associated with Expression of Mutant Protein and Reduced Expression Levels of Embryonic MyHC.PloS one · 2015Article
- Article
- To understand muscle you must take it apart.Frontiers in physiology · 2014Review
- Myosin-II-mediated directional migration of Dictyostelium cells in response to cyclic stretching of substratum.Biophysical journal · 2013Article
- Myosinopathies: pathology and mechanisms.Acta neuropathologica · 2013Review
- Analyses of dynein heavy chain mutations reveal complex interactions between dynein motor domains and cellular dynein functions.Genetics · 2012Article
- Molecular basis of dynamic relocalization of Dictyostelium myosin IB.The Journal of biological chemistry · 2012Article
- Molecular motors: forty years of interdisciplinary research.Molecular biology of the cell · 2011Article
- Drosophila melanogaster myosin-18 represents a highly divergent motor with actin tethering properties.The Journal of biological chemistry · 2011Article
- Myo1c mutations associated with hearing loss cause defects in the interaction with nucleotide and actin.Cellular and molecular life sciences : CMLS · 2011Article
- Nonmuscle myosin IIA with a GFP fused to the N-terminus of the regulatory light chain is regulated normally.Journal of muscle research and cell motility · 2010Article
- The myosin motor domain of fungal chitin synthase V is dispensable for vesicle motility but required for virulence of the maize pathogen Ustilago maydis.The Plant cell · 2010Article
- Structure-based predictive models for allosteric hot spots.PLoS computational biology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We used random mutagenesis to create 21 point mutations in a highly conserved region of the motor domain of Dictyostelium myosin and classified them into three distinct groups based on the ability to complement myosin null cell phenotypes: wild type, intermediate, and null. Biochemical analysis of the mutated myosins also revealed three classes of mutants that correlated well with the phenotypic classification. The mutated myosins that were not fully functional showed defects ranging from ATP nonhydrolyzers to myosins whose enzymatic and mechanical properties are uncoupled. Placement of the mutations onto the three-dimensional structure of myosin showed that the mutated region lay along the cleft that separates the active site from the actin-binding domain and that has been shown to move in response to changes at the active site. These results demonstrate that this region of myosin plays a key role in transduction of chemical energy to mechanical displacement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.