ArticleThe EMBO journal1996
A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- Article
- Interactional similarities and differences in the protein complex of PCNA and DNA replication factor C between rice and Arabidopsis.BMC plant biology · 2019Article
- Somatic Trp53 mutations differentially drive breast cancer and evolution of metastases.Nature communications · 2018Article
- Mechanism of opening a sliding clamp.Nucleic acids research · 2017Article
- Review
- Replication clamps and clamp loaders.Cold Spring Harbor perspectives in biology · 2013Review
- A novel function for the conserved glutamate residue in the walker B motif of replication factor C.Genes · 2013Article
- Keeping chromatin quiet: how nucleosome remodeling restores heterochromatin after replication.Cell cycle (Georgetown, Tex.) · 2011Review
- BRCT domains: easy as one, two, three.Cell cycle (Georgetown, Tex.) · 2011Review
- Loading clamps for DNA replication and repair.DNA repair · 2009Review
- Recruitment of DNA repair synthesis machinery to sites of DNA damage/repair in living human cells.Nucleic acids research · 2007Article
- 1H, 15N and 13C resonance assignments of the BRCT region of the large subunit of human Replication Factor C.Journal of biomolecular NMR · 2005Article
- Dual functions, clamp opening and primer-template recognition, define a key clamp loader subunit.Journal of molecular biology · 2004Article
- Phosphorylation of the PCNA binding domain of the large subunit of replication factor C on Thr506 by cyclin-dependent kinases regulates binding to PCNA.Nucleic acids research · 2003Article
- DNA replication: a complex matter.EMBO reports · 2003Review
- Replication and expansion of trinucleotide repeats in yeast.Molecular and cellular biology · 2003Article
- Regulation of RelA (p65) function by the large subunit of replication factor C.Molecular and cellular biology · 2003Article
- On the specificity of interaction between the Saccharomyces cerevisiae clamp loader replication factor C and primed DNA templates during DNA replication.The Journal of biological chemistry · 2002Article
- A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Replication factor C (RF-C), a complex of five polypeptides, is essential for cell-free SV40 origin-dependent DNA replication and viability in yeast. The cDNA encoding the large subunit of human RF-C (RF-Cp145) was cloned in a Southwestern screen. Using deletion mutants of RF-Cp145 we have mapped the DNA binding domain of RF-Cp145 to amino acid residues 369-480. This domain is conserved among both prokaryotic DNA ligases and eukaryotic poly(ADP-ribose) polymerases and is absent in other subunits of RF-C. The PCNA binding domain maps to amino acid residues 481-728 and is conserved in all five subunits of RF-C. The PCNA binding domain of RF-Cp145 inhibits several functions of RF-C, such as: (i) in vitro DNA replication of SV40 origin-containing DNA; (ii) RF-C-dependent loading of PCNA onto DNA; and (iii) RF-C-dependent DNA elongation. The PCNA binding domain of RF-Cp145 localizes to the nucleus and inhibits DNA synthesis in transfected mammalian cells. In contrast, the DNA binding domain of RF-Cp145 does not inhibit DNA synthesis in vitro or in vivo. We therefore conclude that amino acid residues 481-728 of human RF-Cp145 are critical and act as a dominant negative mutant of RF-C function in DNA replication in vivo.
Indexed as
Identifiers
8861969PMC452166W302805760What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.