ArticleThe EMBO journal1996
Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes.BMC microbiology · 2007Article
- Complete cap 4 formation is not required for viability in Trypanosoma brucei.Eukaryotic cell · 2006Article
- SmD1 is required for spliced leader RNA biogenesis.Eukaryotic cell · 2004Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNA.Eukaryotic cell · 2003Article
- RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons.Genes & development · 2001Article
- trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.Molecular and cellular biology · 2000Article
- Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria.Nucleic acids research · 2000Article
- Alterations in the conserved SL1 trans-spliced leader of Caenorhabditis elegans demonstrate flexibility in length and sequence requirements in vivo.Molecular and cellular biology · 1999Article
- Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.Molecular and cellular biology · 1999Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
- In vivo function of mutated spliced leader RNAs in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In trypanosomes mRNAs are generated through trans splicing. The spliced leader (SL) RNA, which donates the 5'-terminal mini-exon to each of the protein coding exons, plays a central role in the trans splicing process. We have established in vivo assays to study in detail trans splicing, cap4 modification, and RNP assembly of the SL RNA in the trypanosomatid species Leptomonas seymouri. First, we found that extensive sequences within the mini-exon are required for SL RNA function in vivo, although a conserved length of 39 nt is not essential. In contrast, the intron sequence appears to be surprisingly tolerant to mutation; only the stem-loop II structure is indispensable. The asymmetry of the sequence requirements in the stem I region suggests that this domain may exist in different functional conformations. Second, distinct mini-exon sequences outside the modification site are important for efficient cap4 formation. Third, all SL RNA mutations tested allowed core RNP assembly, suggesting flexible requirements for core protein binding. In sum, the results of our mutational analysis provide evidence for a discrete domain structure of the SL RNA and help to explain the strong phylogenetic conservation of the mini-exon sequence and of the overall SL RNA secondary structure; they also suggest that there may be certain differences between trans splicing in nematodes and trypanosomes. This approach provides a basis for studying RNA-RNA interactions in the trans spliceosome.
Indexed as
Identifiers
8861965PMC452162W2194288292What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.