ArticleJournal of molecular neuroscience : MN1995
Human and monkey cholinergic neurons visualized in paraffin-embedded tissues by immunoreactivity for VAChT, the vesicular acetylcholine transporter.
Article in Journal of molecular neuroscience : MN, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Article
- Evaluation of neural innervation in the human conventional outflow pathway distal to Schlemm's canal.Experimental eye research · 2022Review
- Thalamic degeneration in MPTP-treated Parkinsonian monkeys: impact upon glutamatergic innervation of striatal cholinergic interneurons.Brain structure & function · 2019Article
- Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract.Scientific reports · 2019Article
- Molecular Imaging of the Heart.Comprehensive Physiology · 2019Review
- Regional vesicular acetylcholine transporter distribution in human brain: A [The Journal of comparative neurology · 2018Article
- Identification of Functional Cell Groups in the Abducens Nucleus of Monkey and Human by Perineuronal Nets and Choline Acetyltransferase Immunolabeling.Frontiers in neuroanatomy · 2018Article
- Modeling ALS with motor neurons derived from human induced pluripotent stem cells.Nature neuroscience · 2016Review
- Kinetics modeling and occupancy studies of a novel C-11 PET tracer for VAChT in nonhuman primates.Nuclear medicine and biology · 2016Article
- Revisiting enigmatic cortical calretinin-expressing interneurons.Frontiers in neuroanatomy · 2014Review
- Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component.European journal of histochemistry : EJH · 2013Article
- A novel population of cholinergic neurons in the macaque spinal dorsal horn of potential clinical relevance for pain therapy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Inhibition of cholinergic signaling causes apoptosis in human bronchioalveolar carcinoma.Cancer research · 2013Article
- Synthesis and in vitro biological evaluation of carbonyl group-containing inhibitors of vesicular acetylcholine transporter.Journal of medicinal chemistry · 2010Article
- Effects of two years of conjugated equine estrogens on cholinergic neurons in young and middle-aged ovariectomized monkeys.Brain research · 2009Article
- Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Coexpression of cholinergic and noradrenergic phenotypes in human and nonhuman autonomic nervous system.The Journal of comparative neurology · 2005Article
- Elevation of nerve growth factor and antisense knockdown of TrkA receptor during contextual memory consolidation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- A phosphorylation site regulates sorting of the vesicular acetylcholine transporter to dense core vesicles.The Journal of cell biology · 2000Article
- Vesicular neurotransmitter transporters. Potential sites for the regulation of synaptic function.Molecular neurobiology · 1997Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The predicted C-terminal dodecapeptide of the human vesicular acetylcholine transporter (VAChT), deduced from the unique open reading frame of the recently cloned human VAChT cDNA, was conjugated through an N-terminal cysteine to keyhole limpet hemocyanin and used as an immunogen to generate polyclonal antihuman VAChT antibodies in rabbits. The distribution of the VAChT antigen in representative regions of the cholinergic nervous system was examined and compared to that of the acetylcholine biosynthetic enzyme choline acetyltransferase (ChAT), a specific marker for cholinergic neurons. VAChT immunoreactivity was localized in cell bodies of neurons in the basal forebrain and ventral horn of the spinal cord, regions in which major cholinergic projection systems to the cerebral cortex and to skeletal muscle, respectively, originate. The primate caudate nucleus contained numerous VAChT-positive interneurons. VAChT immunoreactivity was visualized in both cell bodies and extensive terminals in striatal interneurons, in contrast to formalin-fixed, deparaffinized sections stained for ChAT, in which cell bodies and fibers were stained but nerve terminals were less well visualized than with the VAChT antiserum. VAChT-positive nerve fibers were visualized in routinely immersion-fixed, paraffin-embedded human cerebral cortex, comparable to the density of fibers observed in perfusion-fixed Bouin's-postfixed monkey cerebral cortex. Extensive investment of virtually all principal ganglion cells of thoracic sympathetic ganglia of monkey and human with VAChT-positive nerve terminals was observed. VAChT-positive cell bodies, presumably corresponding to cholinergic sympathetic sudomotor neurons, were a significant fraction of the total principal cell population in monkey and human thoracic sympathetic ganglia.
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