ArticleThe Journal of cell biology1996
A developmental defect in Plasmodium falciparum male gametogenesis.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Measuring growth, resistance, and recovery after artemisinin treatment of Plasmodium falciparum in a single semi-high-throughput assay.Malaria journal · 2025Article
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- Squaramide Tethered Clindamycin, Chloroquine, and Mortiamide Hybrids: Design, Synthesis, and Antimalarial Activity.ACS medicinal chemistry letters · 2023Article
- Antimalarial Natural Products.Progress in the chemistry of organic natural products · 2022Article
- PfAP2-G2 Is Associated to Production and Maturation of Gametocytes inFrontiers in microbiology · 2020Article
- CRISPR/Cas9 knockouts reveal genetic interaction between strain-transcendent erythrocyte determinants ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Malaria parasite proteins involved in nutrient channels at the host erythrocyte membrane: advances and questions for future research.International journal of current multidisciplinary studies · 2017Article
- Caged Garcinia Xanthones, a Novel Chemical Scaffold with Potent Antimalarial Activity.Antimicrobial agents and chemotherapy · 2017Article
- The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites.PloS one · 2016Article
- Sexual development in Plasmodium parasites: knowing when it's time to commit.Nature reviews. Microbiology · 2015Review
- Sex-partitioning of the Plasmodium falciparum stage V gametocyte proteome provides insight into falciparum-specific cell biology.Molecular & cellular proteomics : MCP · 2014Article
- DNA secondary structures are associated with recombination in major Plasmodium falciparum variable surface antigen gene families.Nucleic acids research · 2014Article
- A framework for assessing the risk of resistance for anti-malarials in development.Malaria journal · 2012Article
- Article
- Gametocytogenesis in malaria parasite: commitment, development and regulation.Future microbiology · 2011Review
- Lack of molecular correlates of Plasmodium vivax ookinete development.The American journal of tropical medicine and hygiene · 2011Article
- Malaria drug resistance is associated with defective DNA mismatch repair.Molecular and biochemical parasitology · 2011Article
- Promiscuous expression of α-tubulin II in maturing male and female Plasmodium falciparum gametocytes.PloS one · 2010Article
- Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum.Molecular biology reports · 2010Article
- Comparative transcriptional and genomic analysis of Plasmodium falciparum field isolates.PLoS pathogens · 2009Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Asexually replicating populations of Plasmodium parasites, including those from cloned lines, generate both male and female gametes to complete the malaria life cycle through the mosquito. The generation of these sexual forms begins with the induction of gametocytes from haploid asexual stage parasites in the blood of the vertebrate host. The molecular processes that govern the differentiation and development of the sexual forms are largely unknown. Here we describe a defect that affects the development of competent male gametocytes from a mutant clone of P. falciparum (Dd2). Comparison of the Dd2 clone to the predecessor clone from which it was derived (W2'82) shows that the defect is a mutation that arose during the long-term cultivation of asexual stages in vitro. Light and electron microscopic images, and indirect immunofluorescence assays with male-specific anti-alpha-tubulin II antibodies, indicate a global disruption of male development at the gametocyte level with at least a 70-90% reduction in the proportion of mature male gametocytes by the Dd2 clone relative to W2'82. A high prevalence of abnormal gametocyte forms, frequently containing multiple and unusually large vacuoles, is associated with the defect. The reduced production of mature male gametocytes may reflect a problem in processes that commit a gametocyte to male development or a progressive attrition of viable male gametocytes during maturation. The defect is genetically linked to an almost complete absence of male gamete production and of infectivity to mosquitoes. This is the first sex-specific developmental mutation identified and characterized in Plasmodium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.