ArticleThe Journal of cell biology1996
Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 94 citations in OpenAlex.
- A centrosome-localized calcium signal is essential for mammalian cell mitosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Remodeling of ER-plasma membrane contact sites but not STIM1 phosphorylation inhibits CaProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Parvoviruses cause nuclear envelope breakdown by activating key enzymes of mitosis.PLoS pathogens · 2013Article
- Intracellular and extracellular pH and Ca are bound to control mitosis in the early sea urchin embryo via ERK and MPF activities.PloS one · 2013Article
- Regulation of store-operated Ca2+ entry during the cell cycle.Journal of cell science · 2010Article
- The influence of bioactive oxylipins from marine diatoms on invertebrate reproduction and development.Marine drugs · 2009Review
- Introduction. Calcium signals and developmental patterning.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Article
- Calcium signalling in early embryos.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Nucleoplasmic calcium is required for cell proliferation.The Journal of biological chemistry · 2007Article
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos.The Journal of cell biology · 2005Article
- Inhibiting MAP kinase activity prevents calcium transients and mitosis entry in early sea urchin embryos.The Journal of biological chemistry · 2005Article
- Ca(2+)(cyt) negatively regulates the initiation of oocyte maturation.The Journal of cell biology · 2004Article
- Activation of protein kinase C alters p34(cdc2) phosphorylation state and kinase activity in early sea urchin embryos by abolishing intracellular Ca2+ transients.The Biochemical journal · 2000Article
- Calcium microdomains and cell cycle control.Cell calciumReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Using calcium-sensitive dyes together with their dextran conjugates and confocal microscopy, we have looked for evidence of localized calcium signaling in the region of the nucleus before entry into mitosis, using the sea urchin egg first mitotic cell cycle as a model. Global calcium transients that appear to originate from the nuclear area are often observed just before nuclear envelope breakdown (NEB). In the absence of global increases in calcium, confocal microscopy using Calcium Green-1 dextran indicator dye revealed localized calcium transients in the perinuclear region. We have also used a photoinactivatable calcium chelator, nitrophenyl EGTA (NP-EGTA), to test whether the chelator-induced block of mitosis entry can be reversed after inactivation of the chelator. Cells arrested before NEB by injection of NP-EGTA resume the cell cycle after flash photolysis of the chelator. Photolysis of chelator triggers calcium release. TreatmenT with caFfeine to enhance calcium-induced calcium release increases the amplitude of NEB-associated calcium transients. These results indicate that calcium increases local to the nucleus are required to trigger entry into mitosis. Local calcium transients arise in the perinuclear region and can spread from this region into the cytoplasm. Thus, cell cycle calcium signals are generated by the perinuclear mitotic machinery in early sea urchin embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.