ArticleThe Journal of cell biology1996
Apical and basolateral endosomes of MDCK cells are interconnected and contain a polarized sorting mechanism.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 135 citations in OpenAlex.
- Differential compartmentalization of BMP4/NOGGIN requires NOGGIN trans-epithelial transport.Developmental cell · 2021Article
- Membrane Transport across Polarized Epithelia.Cold Spring Harbor perspectives in biology · 2017Review
- A targeted RNAi screen identifies factors affecting diverse stages of receptor-mediated transcytosis.The Journal of cell biology · 2017Article
- Endosome-mitochondria interactions are modulated by iron release from transferrin.The Journal of cell biology · 2016Article
- Iterative sorting of apical and basolateral cargo in Madin-Darby canine kidney cells.Molecular biology of the cell · 2016Article
- Prion protein promotes kidney iron uptake via its ferrireductase activity.The Journal of biological chemistry · 2015Article
- Spatially restricted G protein-coupled receptor activity via divergent endocytic compartments.The Journal of biological chemistry · 2014Article
- Ganglioside GM1-mediated transcytosis of cholera toxin bypasses the retrograde pathway and depends on the structure of the ceramide domain.The Journal of biological chemistry · 2013Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells.Developmental cell · 2012Article
- Helicobacter pylori perturbs iron trafficking in the epithelium to grow on the cell surface.PLoS pathogens · 2011Article
- Genetic dissection reveals unexpected influence of beta subunits on KCNQ1 K+ channel polarized trafficking in vivo.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Tyrosine-dependent basolateral targeting of human connexin43-eYFP in Madin-Darby canine kidney cells can be disrupted by the oculodentodigital dysplasia mutation L90V.The FEBS journal · 2009Article
- Early steps in cell infection by parvoviruses: host-specific differences in cell receptor binding but similar endosomal trafficking.Journal of virology · 2009Article
- The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity.The Journal of cell biology · 2009Article
- Receptor complexes cotransported via polarized endocytic pathways form clusters with distinct organizations.Molecular biology of the cell · 2007Article
- Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin1.The Journal of cell biology · 2006Article
- Antibody-specific detection of caveolin-1 in subapical compartments of MDCK cells.Histochemistry and cell biology · 2006Article
- Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2006Article
- Functional interaction between p75NTR and TrkA: the endocytic trafficking of p75NTR is driven by TrkA and regulates TrkA-mediated signalling.The Biochemical journal · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have evaluated transcytotic routes in MDCK cells for their ability to generate a polarized surface distribution of trafficking proteins by following the intracellular sorting of transferrin receptors (TRs). We find that the selective basolateral expression of TRs is maintained in the face of extensive trafficking between the apical and basolateral surfaces. Biochemical studies of receptors loaded with tracer under conditions approaching steady state indicate that TRs internalized from the two surfaces are extensively colocalized within MDCK cells and that both populations of receptors are selectively delivered to the basolateral surface. Tailless TRs in which the cytoplasmic domain has been deleted display an unpolarized cell surface distribution and recycle in an unpolarized fashion. We show by EM that wild-type receptors internalized from each surface are colocalized within endosomal elements distributed throughout the cytoplasm. By preloading endosomal elements directly accessible from the basolateral surface with transferrin (Tf)-HRP, we show that apically internalized TRs rapidly enter the same compartment. We also show that both transcytosing (apically internalized) and recycling (basolaterally internalized) TRs are delivered to the basolateral border by a distinctive subset of exocytotic, 60-nm-diam vesicles. Together, the biochemical and morphological data show that apical and basolateral endosomes of MDCK cells are interconnected and contain a signal-dependent polarized sorting mechanism. We propose a dynamic model of polarized sorting in MDCK cells in which a single endosome-based, signal-dependent sorting step is sufficient to maintain the polarized phenotype.
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