ArticleMolecular biology of the cell1996
Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 140 citations in OpenAlex.
- Immunoglobulin κ drives liver regeneration by stabilizing MYLK to maintain cytoskeletal integrity and promote hepatocyte proliferation.Journal of translational internal medicine · 2026Article
- Did axons evolve by activating cytokinesis during interphase? A hypothesis on the origin of neurons.Molecular biology of the cell · 2025Review
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.bioRxiv : the preprint server for biology · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.Frontiers in cell and developmental biology · 2024Article
- Early zygotic gene product Dunk interacts with anillin to regulate Myosin II duringMolecular biology of the cell · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
- Biomedical Applications of Translational Optical Imaging: From Molecules to Humans.Molecules (Basel, Switzerland) · 2021Review
- Polar relaxation by dynein-mediated removal of cortical myosin II.The Journal of cell biology · 2020Article
- Cortical contraction drives the 3D patterning of epithelial cell surfaces.The Journal of cell biology · 2020Article
- The balance between adhesion and contraction during cell division.Current opinion in cell biology · 2019Review
- A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis ineLife · 2018Article
- Asymmetric Flows in the Intercellular Membrane during Cytokinesis.Biophysical journal · 2017Article
- Article
- Myosin efflux promotes cell elongation to coordinate chromosome segregation with cell cleavage.Nature communications · 2017Article
- The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis.Molecular biology of the cell · 2017Article
- Flow-dependent myosin recruitment during Drosophila cellularization requires zygotic dunk activity.Development (Cambridge, England) · 2016Article
- Actin binding domain of filamin distinguishes posterior from anterior actin filaments in migratingBiophysics and physicobiology · 2016Article
- Non-muscle myosin II in disease: mechanisms and therapeutic opportunities.Disease models & mechanisms · 2015Review
- Article
- Fluorescent protein biosensors applied to microphysiological systems.Experimental biology and medicine (Maywood, N.J.) · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The mechanism of cytokinesis has been difficult to define because of the short duration and the temporal-spatial dynamics involved in the formation, activation, force production, and disappearance of the cleavage furrow. We have investigated the structural and chemical dynamics of myosin II in living Swiss 3T3 cells from prometaphase through the separation and migration of daughter cells. The structural and chemical dynamics of myosin II have been defined using the semiautomated, multimode light microscope, together with a fluorescent analogue of myosin II and a fluorescent biosensor of myosin II regulatory light chain (RLC) phosphorylation at serine 19. The correlation of image data from live cells using different modes of light microscopy allowed interpretations not possible from single-mode investigations. Myosin II transported toward the equatorial plane from adjacent regions, forming three-dimensional fibers that spanned the volume of the equator during anaphase and telophase. A global phosphorylation of myosin II at serine 19 of the RLC was initiated at anaphase when cortical myosin II transport started. The phosphorylation of myosin II remained high near the equatorial plane through telophase and into cytokinesis, whereas the phosphorylation of myosin II at serine 19 of the RLC decreased at the poles. The timing and pattern of phosphorylation was the same as the shortening of myosin II-based fibers in the cleavage furrow. Myosin II-based fibers shortened and transported out of the cleavage furrow into the tails of the two daughter cells late in cytokinesis. The patterns of myosin II transport, phosphorylation, and shortening of fibers in the migrating daughter cells were similar to that previously defined for cells migrating in a wound in vitro. The temporal-spatial patterns and dynamics of myosin II transport, phosphorylation at serine 19 of the RLC, and the shortening and disappearance of myosin II-based fibers support the proposal that a combination of the cortical flow hypothesis and the solation-contraction coupling hypothesis explain key aspects of cytokinesis and polarized cell locomotion.
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Registered trials
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