ArticleProceedings of the National Academy of Sciences of the United States of America1996
Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 190 citations in OpenAlex.
- Possible Involvement of Lysophospholipids in Severe Asthma as Novel Lipid Mediators.Biomolecules · 2025Review
- The Role of Chemokines in Orchestrating the Immune Response to Pancreatic Ductal Adenocarcinoma.Cancers · 2024Review
- Review
- Chemokines and their receptors: predictors of the therapeutic potential of mesenchymal stromal cells.Journal of translational medicine · 2021Review
- Macrophage Migration and Phagocytosis Are Controlled by Kindlin-3's Link to the Cytoskeleton.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Toll-like receptor 2 (TLR2) induces migration and invasive mechanisms in rheumatoid arthritis.Arthritis research & therapy · 2015Article
- Helicobacter pylori outer membrane vesicle proteins induce human eosinophil degranulation via a β2 Integrin CD11/CD18- and ICAM-1-dependent mechanism.Mediators of inflammation · 2015Article
- Activation states of blood eosinophils in asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2014Review
- Inflammatory cell trafficking across the blood-brain barrier: chemokine regulation and in vitro models.Immunological reviews · 2012Review
- Role of very long fatty acid-containing glycosphingolipids in membrane organization and cell signaling: the model of lactosylceramide in neutrophils.Glycoconjugate journal · 2009Review
- Selective human endothelial cell activation by chemokines as a guide to cell homing.Immunology · 2009Article
- Proline-rich tyrosine kinase 2 regulates spreading and migration of eosinophils after beta2-integrin adhesion.American journal of respiratory cell and molecular biology · 2008Article
- Roles of integrin activation in eosinophil function and the eosinophilic inflammation of asthma.Journal of leukocyte biology · 2008Review
- Article
- Mechanisms of eosinophilia in the pathogenesis of hypereosinophilic disorders.Immunology and allergy clinics of North America · 2007Review
- Regulation of eosinophil adhesion by lysophosphatidylcholine via a non-store-operated Ca2+ channel.American journal of respiratory cell and molecular biology · 2007Article
- Inflammatory cell migration into the central nervous system: a few new twists on an old tale.Brain pathology (Zurich, Switzerland) · 2007Review
- Dissection of the hyperadhesive phenotype of airway eosinophils in asthma.American journal of respiratory cell and molecular biology · 2006Article
- Regulation of immune cell adhesion and migration by regulator of adhesion and cell polarization enriched in lymphoid tissues.Immunology · 2005Review
- Eosinophil function in allergic inflammation: from bone marrow to tissue response.Current allergy and asthma reports · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The CC chemokines regulated on activation normal T expressed and secreted (RANTES) and monocyte chemotactic protein 3 (MCP-3), and the anaphylatoxin C5a, induce activation, degranulation, chemotaxis, and transendothelial migration of eosinophils. Adhesion assays on purified ligands showed differential regulation of beta 1 and beta 2 integrin avidity in eosinophils. Adhesiveness of VLA-4 (alpha 4 beta 1, CD29/CD49d) for vascular cell adhesion molecule 1 or fibronectin was rapidly increased but subsequently reduced by RANTES, MCP-3, or C5a. The deactivation of VLA-4 lead to cell detachment, whereas phorbol 12-myristate 13-acetate induced sustained activation of VLA-4. In contrast, chemoattractants stimulated a prolonged increase in the adhesiveness of Mac-1 (alpha M beta 2, CD11b/CD18) for intercellular adhesion molecule 1. Inhibition by pertussis toxin confirmed signaling via G protein-coupled receptors. Chemoattractants induced transient, while phorbol 12-myristate 13-acetate induced sustained actin polymerization. Disruption of actin filaments by cytochalasins inhibited increases in avidity of VLA-4 but not of Mac-1. Chemoattractants did not upregulate a Mn2+-inducible beta 1 neoepitope defined by the mAb 9EG7, but induced prolonged expression of a Mac-1 activation epitope recognized by the mAb CBRM1/5. This mAb inhibited chemoattractant-stimulated adhesion of eosinophils to intercellular adhesion molecule 1. Thus, regulation of VLA-4 was dependent on the actin cytoskeleton, whereas conformational changes appeared to be crucial for activation of Mac-1. To our knowledge, this is the first demonstration that physiological agonists, such as chemoattractants, can differentially regulate the avidity of a beta 1 and a beta 2 integrin expressed on the same leukocyte.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.