ArticleGenetics1996
Copy number and orientation determine the susceptibility of a gene to silencing by nearby heterochromatin in Drosophila.
Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 95 citations in OpenAlex.
- The sound of silence: Transgene silencing in mammalian cell engineering.Cell systems · 2022Review
- Species-specific chromatin landscape determines how transposable elements shape genome evolution.eLife · 2022Article
- The Coming Age of Insect Cells for Manufacturing and Development of Protein Therapeutics.Industrial & engineering chemistry research · 2018Article
- Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs.Transgenic research · 2017Article
- Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.PLoS neglected tropical diseases · 2014Article
- Molecular characterization of transgene integration by next-generation sequencing in transgenic cattle.PloS one · 2012Article
- Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation.Gene · 2010Article
- Gene structure induced epigenetic modifications of pericarp color1 alleles of maize result in tissue-specific mosaicism.PloS one · 2009Article
- Stability and loss of a virus resistance phenotype over time in transgenic mosquitoes harbouring an antiviral effector gene.Insect molecular biology · 2009Article
- Article
- Orientation-dependent regulation of integrated HIV-1 expression by host gene transcriptional readthrough.Cell host & microbe · 2008Article
- Position effect variegation and imprinting of transgenes in lymphocytes.Nucleic acids research · 2008Article
- A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein.Genesis (New York, N.Y. : 2000) · 2007Article
- Genome-wide HP1 binding in Drosophila: developmental plasticity and genomic targeting signals.Genome research · 2005Article
- The human beta-globin locus control region can silence as well as activate gene expression.Molecular and cellular biology · 2005Article
- Article
- Effect of the Suppressor of Underreplication (SuUR) gene on position-effect variegation silencing in Drosophila melanogaster.Genetics · 2003Article
- Article
- Position effects are influenced by the orientation of a transgene with respect to flanking chromatin.Molecular and cellular biology · 2001Article
- Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced.Genes & development · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The classical phenomenon of position-effect variegation (PEV) is the mosaic expression that occurs when a chromosomal rearrangement moves a euchromatic gene near heterochromatin. A striking feature of this phenomenon is that genes far away from the junction with heterochromatin can be affected, as if the heterochromatic state "spreads." We have investigated classical PEV of a Drosophila brown transgene affected by a heterochromatic junction approximately 60 kb away. PEV was enhanced when the transgene was locally duplicated using P transposase. Successive rounds of P transposase mutagenesis and phenotypic selection produced a series of PEV alleles with differences in phenotype that depended on transgene copy number and orientation. As for other examples of classical PEV, nearby heterochromatin was required for gene silencing. Modifications of classical PEV by alterations at a single site are unexpected, and these observations contradict models for spreading that invoke propagation of heterochromatin along the chromosome. Rather, our results support a model in which local alterations affect the affinity of a gene region for nearby heterochromatin via homology-based pairing, suggesting an alternative explanation for this 65-year-old phenomenon.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.