ArticleAntimicrobial agents and chemotherapy1996
Molecular characterization of the BRO beta-lactamase of Moraxella (Branhamella) catarrhalis.
Article in Antimicrobial agents and chemotherapy, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- The bovine ocular microbiome: a multi-approach study of composition and antimicrobial activity.Animal microbiome · 2026Article
- Investigating the population structure of Moraxella catarrhalis using a cgMLST scheme and LIN code system.Nature communications · 2025Article
- Understanding the population structure ofbioRxiv : the preprint server for biology · 2025Article
- OXA β-lactamases frommBio · 2025Article
- OXA β-lactamases frombioRxiv : the preprint server for biology · 2024Article
- The biogenesis of β-lactamase enzymes.Microbiology (Reading, England) · 2022Review
- The characterization ofMicrobial genomics · 2022Article
- Mechanisms Involved in the Active Secretion of CTX-M-15 β-Lactamase by Pathogenic Escherichia coli ST131.Antimicrobial agents and chemotherapy · 2021Article
- Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.Chemical reviews · 2021Review
- Article
- Membrane anchoring stabilizes and favors secretion of New Delhi metallo-β-lactamase.Nature chemical biology · 2016Article
- Comparative Genomic Analyses of the Moraxella catarrhalis Serosensitive and Seroresistant Lineages Demonstrate Their Independent Evolution.Genome biology and evolution · 2016Article
- Novel Moraxella catarrhalis prophages display hyperconserved non-structural genes despite their genomic diversity.BMC genomics · 2015Article
- Residence of Streptococcus pneumoniae and Moraxella catarrhalis within polymicrobial biofilm promotes antibiotic resistance and bacterial persistence in vivo.Pathogens and disease · 2014Article
- RNA-Seq-based analysis of the physiologic cold shock-induced changes in Moraxella catarrhalis gene expression.PloS one · 2013Article
- Mobile genetic elements in Moraxella catarrhalis.Mobile genetic elements · 2011Article
- Down-regulation of porin M35 in Moraxella catarrhalis by aminopenicillins and environmental factors and its potential contribution to the mechanism of resistance to aminopenicillins.The Journal of antimicrobial chemotherapy · 2010Article
- Genome analysis of Moraxella catarrhalis strain BBH18, [corrected] a human respiratory tract pathogen.Journal of bacteriology · 2010Article
- Molecular aspects of Moraxella catarrhalis pathogenesis.Microbiology and molecular biology reviews : MMBR · 2009Review
- Contemporary prevalence of BRO beta-lactamases in Moraxella catarrhalis: report from the SENTRY antimicrobial surveillance program (North America, 1997 to 2004).Journal of clinical microbiology · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid increase in the prevalence of beta-lactamase-producing Moraxella (Branhamella) catarrhalis strains has been noticed during the last decades. Today, more than 80% of strains isolated worldwide produce beta-lactamase. To investigate beta-lactamase(s) of M. catarrhalis at the molecular level, the BRO-1 beta-lactamase gene (bla) was isolated as part of a 4,223-bp HindIII fragment. Sequence analysis indicated that bla encodes a polypeptide of 314 amino acid residues. Insertional inactivation of bla in M. catarrhalis resulted in complete abrogation of beta-lactamase production and ampicillin resistance, demonstrating that bla is solely responsible for beta-lactam resistance. Comparison with other beta-lactamases suggested that M. catarrhalis beta-lactamase is a unique enzyme with conserved residues at the active sites. The presence of a signal sequence for lipoproteins suggested that it is lipid modified at its N terminus. In keeping with this assumption was the observation that 10% of beta-lactamase activity was found in the membrane compartment of M. catarrhalis. M. catarrhalis strains produce two types of beta-lactamase, BRO-1 and BRO-2, which differ in their isoelectric points. The BRO-1 and BRO-2 genes from two ATCC strains of M. catarrhalis were sequenced, and only one amino acid difference was found between the predicted products. However, there was a 21-bp deletion in the promoter region of the BRO-2 gene, possibly explaining the lower level of production of BRO-2. The G + C content of bla (31%) was significantly lower than those of the flanking genes (47 and 50%), and the overall G + C content of the M. catarrhalis genome (41%). These results indicate that bla was acquired by horizontal gene transfer from another, still unknown species.
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