ArticleThe EMBO journal1995
Cadaverine induces closing of E. coli porins.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 87 citations in OpenAlex.
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- Adaptive laboratory evolution ofMicrobiology (Reading, England) · 2020Article
- Genome-scale model of metabolism and gene expression provides a multi-scale description of acid stress responses in Escherichia coli.PLoS computational biology · 2019Article
- A Novel Process for Cadaverine Bio-Production Using a Consortium of Two EngineeredFrontiers in microbiology · 2018Article
- Molecular and proteome analyses highlight the importance of the Cpx envelope stress system for acid stress and cell wall stability in Escherichia coli.MicrobiologyOpen · 2016Article
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- The role of transport mechanisms in mycobacterium tuberculosis drug resistance and tolerance.Pharmaceuticals (Basel, Switzerland) · 2012Article
- MspA nanopores from subunit dimers.PloS one · 2012Article
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- Elucidating in vivo structural dynamics in integral membrane protein by hydroxyl radical footprinting.Molecular & cellular proteomics : MCP · 2009Article
- Outer membrane permeability and antibiotic resistance.Biochimica et biophysica acta · 2009Review
- Polyamines improve K+/Na+ homeostasis in barley seedlings by regulating root ion channel activities.Plant physiology · 2007Article
- Polyamine stress at high pH in Escherichia coli K-12.BMC microbiology · 2005Article
- Molecular basis of bacterial outer membrane permeability revisited.Microbiology and molecular biology reviews : MMBR · 2003Review
- Colicins, spermine and cephalosporins: a competitive interaction with the OmpF eyelet.The Biochemical journal · 2003Article
- Cadaverine inhibition of porin plays a role in cell survival at acidic pH.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have used the electrophysiological technique of patch-clamp to study the modulation of Escherichia coli porins by cadaverine. Porin channels typically have a very high probability to be open, and were not known to be inhibited by specific compounds until the present study. Experiments performed on patches of outer membrane reconstituted in liposomes reveal that cadaverine applied to the periplasmic side increases the frequency of channel closures in a concentration-dependent fashion, and thereby decreases the total amount of ion flux through a porin-containing membrane. The positive charge on cadaverine is important for inhibition, because the effect is relieved at higher pH where fewer polyamine molecules are charged. Modulation is observed only at negative pipet voltages, and therefore confers voltage dependence to porin activity. Cadaverine increases the number and duration of cooperative closures of more than one channel, suggesting that it does not merely block the pore but exerts its kinetic effect allosterically. As a biological assay of porin inhibition, E. coli behavior in chemotaxis swarm plates was tested and found to be impaired in the presence of cadaverine. Polyamines are naturally found associated with the outer membrane of E.coli, but are lost upon fractionation. We postulate that cadaverine might be a natural regulator of porin activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.