ArticleThe EMBO journal1995
Physical separation of two different forms of human TFIIIB active in the transcription of the U6 or the VAI gene in vitro.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- RNA Polymerase III Subunit Mutations in Genetic Diseases.Frontiers in molecular biosciences · 2021Review
- Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements.Cell cycle (Georgetown, Tex.) · 2018Article
- Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.Transcription · 2014Review
- Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells.Nucleic acids research · 2012Article
- Genomic relationship between SINE retrotransposons, Pol III-Pol II transcription, and chromatin organization: the journey from junk to jewel.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2011Review
- Different sequence signatures in the upstream regions of plant and animal tRNA genes shape distinct modes of regulation.Nucleic acids research · 2011Article
- Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.Nature structural & molecular biology · 2011Article
- General transcription factors and subunits of RNA polymerase III: Paralogs for promoter- and cell type-specific transcription in multicellular eukaryotes.Transcription · 2010Article
- Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.Cell cycle (Georgetown, Tex.) · 2010Review
- Two isoforms of human RNA polymerase III with specific functions in cell growth and transformation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Protein interactions involved in tRNA gene-specific integration of Dictyostelium discoideum non-long terminal repeat retrotransposon TRE5-A.Molecular and cellular biology · 2007Article
- Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene.Nucleic acids research · 2003Article
- Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing.Molecular and cellular biology · 2002Article
- Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.Nucleic acids research · 2001Review
- Development of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein.Nucleic acids research · 2001Article
- A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription.Molecular and cellular biology · 2000Article
- Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.Genes & development · 2000Article
- Transcription efficiency of human polymerase III genes in vitro does not depend on the RNP-forming autoantigen La.Nucleic acids research · 2000Article
- Alternatively spliced hBRF variants function at different RNA polymerase III promoters.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human transcription factor hTFIIIB is necessary to initiate transcription correctly from all RNA polymerase III (pol III) genes which are governed by structurally different promoters, and it is unclear whether hTFIIIB complexes, required for intragenic or 5'-located pol III promoters, are composed of unique or different components. We show here that two different forms of hTFIIIB can be separated physically by ion exchange chromatography. hTFIIIB-alpha shows strong preference for transcription of the U6 over the VAI gene and does not contain TATA binding protein (TBP). After SDS-PAGE and renaturation of proteins, the transcriptional activity of hTFIIIB-alpha can be reconstituted by fractions corresponding to a mean M(r) of 25, 60 and 90 kDa. Upon gradient centrifugation or gel filtration, the activity of hTFIIIB-alpha is associated with an M(r) of 60 +/- 10 kDa, indicating that the components of the complex tend to dissociate. In contrast, hTFIIIB-beta is predominantly active on intragenic pol III promoters. It reveals an M(r) of 300 +/- 30 kDa upon gel filtration and, besides TBP, it contains several associated factors (TAFs). Two of these proteins reveal an M(r) of 60 kDa and 90 kDa, and it is conceivable that they are related to polypeptides of similar mass functionally identified in hTFIIIB-alpha. These protein are probably required for the recruitment of pol III to the initiation site at 5'-located and intragenic promoters.
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