ArticleThe EMBO journal1995
Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 162 citations in OpenAlex.
- Constitutively active RAS prolongs Cdc42 signalling, while MAPK signalling is attenuated during fission yeast mating.PLoS genetics · 2026Article
- Distinct Cdc42 protein levels differentially regulate polarized growth and cell fusion in Schizosaccharomyces pombe.PLoS biology · 2026Article
- Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.The Journal of cell biology · 2024Article
- The Arp2/3 complex promotes periodic removal of Pak1-mediated negative feedback to facilitate anticorrelated Cdc42 oscillations.bioRxiv : the preprint server for biology · 2023Article
- Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.Journal of cell science · 2023Article
- Article
- Review
- Phosphoregulation of the cytokinetic protein Fic1 contributes to fission yeast growth polarity establishment.Journal of cell science · 2020Article
- Small-GTPase-associated signaling by the guanine nucleotide exchange factors CpDock180 and CpCdc24, the GTPase effector CpSte20, and the scaffold protein CpBem1 in Claviceps purpurea.Eukaryotic cell · 2014Article
- The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.PloS one · 2013Article
- Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth.Science (New York, N.Y.) · 2012Article
- PAK family kinases: Physiological roles and regulation.Cellular logistics · 2012Article
- Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.Journal of cell science · 2011Article
- Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.The Journal of biological chemistry · 2010Article
- Schizosaccharomyces pombe Pak-related protein, Pak1p/Orb2p, phosphorylates myosin regulatory light chain to inhibit cytokinesis.The Journal of cell biology · 2008Article
- Localized activation of p21-activated kinase controls neuronal polarity and morphology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p.Molecular biology of the cell · 2007Article
- Ras GTPase-activating protein gap1 of the homobasidiomycete Schizophyllum commune regulates hyphal growth orientation and sexual development.Eukaryotic cell · 2006Article
- Article
- The novel fission yeast protein Pal1p interacts with Hip1-related Sla2p/End4p and is involved in cellular morphogenesis.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A STE20/p65pak homolog was isolated from fission yeast by PCR. The pak1+ gene encodes a 72 kDa protein containing a putative p21-binding domain near its amino-terminus and a serine/threonine kinase domain near its carboxyl-terminus. The Pak1 protein autophosphorylates on serine residues and preferentially binds to activated Cdc42p both in vitro and in vivo. This binding is mediated through the p21 binding domain on Pak1p and the effector domain on Cdc42p. Overexpression of an inactive mutant form of pak1 gives rise to cells with markedly abnormal shape with mislocalized actin staining. Pak1 overexpression does not, however, suppress lethality associated with cdc42-null cells or the morphologic defeat caused by overexpression of mutant cdc42 alleles. Gene disruption of pak1+ establishes that, like cdc42+, pak1+ function is required for cell viability. In budding yeast, pak1+ expression restores mating function to STE20-null cells and, in fission yeast, overexpression of an inactive form of Pak inhibits mating. These results indicate that the Pak1 protein is likely to be an effector for Cdc42p or a related GTPase, and suggest that Pak1p is involved in the maintenance of cell polarity and in mating.
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