ArticleThe EMBO journal1995
Identification of two novel arginine binding DNAs.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 140 citations in OpenAlex.
- Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells.Nature protocols · 2025Review
- The Application of Aptamer and Research Progress in Liver Disease.Molecular biotechnology · 2024Review
- Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins.Communications biology · 2024Article
- Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.Nucleic acids research · 2023Article
- A system for multiplexed selection of aptamers with exquisite specificity without counterselection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Development of Structure-Switching Aptamers for Kanamycin Detection Based on Fluorescence Resonance Energy Transfer.Frontiers in chemistry · 2019Article
- Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization.Nucleic acids research · 2018Article
- An Integrated Microfluidic SELEX Approach Using Combined Electrokinetic and Hydrodynamic Manipulation.SLAS technology · 2017Article
- INTEGRATED MICROFLUIDIC SELEX USING FREE SOLUTION ELECTROKINETICS.Journal of the Electrochemical Society · 2017Article
- Selection and Biosensor Application of Aptamers for Small Molecules.Frontiers in chemistry · 2016Review
- Computational docking simulations of a DNA-aptamer for argininamide and related ligands.Journal of computer-aided molecular design · 2015Article
- C-STrap Sample Preparation Method--In-Situ Cysteinyl Peptide Capture for Bottom-Up Proteomics Analysis in the STrap Format.PloS one · 2015Article
- Homoiterons and expansion in ribosomal RNAs.FEBS open bio · 2015Article
- Clinical applications of nucleic acid aptamers in cancer.Molecular and clinical oncology · 2014Review
- New strategies for evaluation and analysis of SELEX experiments.BioMed research international · 2014Article
- Aptamers: novel molecules as diagnostic markers in bacterial and viral infections?BioMed research international · 2013Review
- Cotinine-conjugated aptamer/anti-cotinine antibody complexes as a novel affinity unit for use in biological assays.Experimental & molecular medicine · 2012Article
- Challenges and opportunities for small molecule aptamer development.Journal of nucleic acids · 2012Article
- Cell-specific aptamers as emerging therapeutics.Journal of nucleic acids · 2011Article
- Development of DNA aptamers using Cell-SELEX.Nature protocols · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA tertiary structure is known to play critical roles in RNA-protein recognition and RNA function. To examine how DNA tertiary structure might relate to RNA structure, we performed in vitro selection experiments to identify single-stranded DNAs that specifically bind arginine, and compared the results with analogous experiments performed with RNA. In the case of RNA, a motif related to the arginine binding site in human immunodeficiency virus TAR RNA was commonly found, whereas in the case of DNA, two novel motifs and no TAR-like structures were found. One DNA motif, found in approximately 40% of the cloned sequences, forms of hairpin structure with a highly conserved 10 nucleotide loop, whereas the second motif is especially rich in G residues. Chemical interference and mutagenesis experiments identified nucleotides in both motifs that form specific arginine binding sites, and dimethylsulfate footprinting experiments identified single guanine residues in both that are protected from methylation in the presence of arginine, suggesting possible sites of arginine contact or conformational changes in the DNAs. Circular dichroism experiments indicated that both DNAs undergo conformational changes upon arginine binding and that the arginine guanidinium group alone is responsible for binding. A model for the G-rich motif is proposed in which mixed guanine and adenine quartets may form a novel DNA structure. Arginine binding DNAs and RNAs should provide useful model systems for studying nucleic acid tertiary structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.