ArticlePlant molecular biology1996
Isolation and characterization of cDNAs encoding vacuolar H(+)-pyrophosphatase isoforms from rice (Oryza sativa L.).
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- BnVP1, a novel vacuolar H+ pyrophosphatase gene from Boehmeria nivea confers cadmium tolerance in transgenic Arabidopsis.PloS one · 2024Article
- Proton-pumping pyrophosphatase homeolog expression is a dynamic trait in bread wheat (Plant direct · 2021Article
- High V-PPase activity is beneficial under high salt loads, but detrimental without salinity.The New phytologist · 2018Article
- Regulation of Na+ and K+ homeostasis in plants: towards improved salt stress tolerance in crop plants.Genetics and molecular biology · 2017Article
- Characterization and expression analyses of the H⁺-pyrophosphatase gene in rye.Journal of genetics · 2016Article
- Physiological and molecular mechanisms of plant salt tolerance.Photosynthesis research · 2013Review
- Ion Transporters and Abiotic Stress Tolerance in Plants.ISRN molecular biology · 2012Review
- Expression of vacuolar H+-pyrophosphatase (OVP3) is under control of an anoxia-inducible promoter in rice.Plant molecular biology · 2010Article
- Vacuolar H+-translocating inorganic pyrophosphatase (Vpp1) marks partial aleurone cell fate in cereal endosperm development.Plant molecular biology · 2004Article
- Diethylpyrocarbonate inhibition of vacuolar H+-pyrophosphatase possibly involves a histidine residue.Journal of protein chemistry · 2002Article
- AVP2, a sequence-divergent, K(+)-insensitive H(+)-translocating inorganic pyrophosphatase from Arabidopsis.Plant physiology · 2000Article
- Localization of a carboxylic residue possibly involved in the inhibition of vacuolar H+-pyrophosphatase by N, N'-dicyclohexylcarbodi-imide.The Biochemical journal · 1999Article
- Structural aspects of the effectiveness of bisphosphonates as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase.The Biochemical journal · 1999Article
- Article
- The molecular basis of C4 photosynthesis in sorghum: isolation, characterization and RFLP mapping of mesophyll- and bundle-sheath-specific cDNAs obtained by differential screening.Plant molecular biology · 1998Article
- Subunit interaction of vacuolar H+-pyrophosphatase as determined by high hydrostatic pressure.The Biochemical journal · 1998Article
- Molecular cloning of vacuolar H(+)-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean.Plant physiology · 1998Article
- Tris Is a Competitive Inhibitor of K+ Activation of the Vacuolar H+-Pumping Pyrophosphatase.Plant physiology · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vacuolar H(+)-pyrophosphatase (V-PPase) is an electrogenic H+ pump, which was found in the plant vacuolar membrane. Two cDNA clones (OVP1 and OVP2) encoding the V-PPase were isolated from cultured rice (Oryza sativa L.) cells and subsequently sequenced. The sequence analysis has revealed that OVP1 contains 2316 nucleotides of open reading frame (ORF) and 362 nucleotides of the 3'-untranslated region, whereas OVP2 comprises 2304 nucleotides of ORF and 312 nucleotides of the 3'-untranslated region. The nucleotide sequences of ORF of OVP1 and OVP2 are 80.7% identical, and their 5'- and 3'-untranslated regions have 39.4% and 48.4% identity, respectively. The polypeptides encoded by the ORF of OVP1 and OVP2 contain 771 and 767 amino acids, respectively, and the sequences of the OVP proteins are very similar to those of other V-PPases, which are shown to have 85-91% homology. Chromosomal mapping by RFLP techniques demonstrates that OVP1 and OVP2 are isoforms encoded by different genes. Both OVP1 and OVP2 are mapped on the same chromosome (chromosome 6) to a distance of ca.90 cM. Northern analysis indicates that the OVP1 and OVP2 are also expressed in intact rice plants and OVP2 shows higher expression in the calli than the roots and shoots, compared to OVP1. These results show that at least two genes encoding the V-PPases are present in rice genome and their expressions are probably regulated in a different manner.
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