ArticleBiophysical journal1996
Simultaneous capacitance and amperometric measurements of exocytosis: a comparison.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 76 citations in OpenAlex.
- Combined electrochemistry and mass spectrometry imaging to interrogate the mechanism of action of modafinil, a cognition-enhancing drug, at the cellular and sub-cellular level.QRB discovery · 2021Article
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Expanding neurochemical investigations with multi-modal recording: simultaneous fast-scan cyclic voltammetry, iontophoresis, and patch clamp measurements.The Analyst · 2016Article
- TRPM7 kinase activity regulates murine mast cell degranulation.The Journal of physiology · 2016Article
- Mechanisms of granule membrane recapture following exocytosis in intact mast cells.The Journal of biological chemistry · 2013Article
- Single cell amperometry reveals glycocalyx hinders the release of neurotransmitters during exocytosis.Analytical chemistry · 2013Article
- High resolution electrophysiological techniques for the study of calcium-activated exocytosis.Biochimica et biophysica acta · 2012Review
- Unperturbed islet α-cell function examined in mouse pancreas tissue slices.The Journal of physiology · 2011Article
- Fusion gains independence.The Journal of general physiology · 2008Article
- Different effects on fast exocytosis induced by synaptotagmin 1 and 2 isoforms and abundance but not by phosphorylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Formation, stabilisation and fusion of the readily releasable pool of secretory vesicles.Pflugers Archiv : European journal of physiology · 2004Review
- Stimulation of Ca(2+)-independent exocytosis in rat pituitary gonadotrophs by G-protein.The Journal of physiology · 2000Article
- Quantal analysis of 5-hydroxytryptamine release from mouse pancreatic beta-cells.The Journal of physiology · 1999Article
- Multiple kinetic components and the Ca2+ requirements of exocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
- Post-priming actions of ATP on Ca2+-dependent exocytosis in pancreatic beta cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Comparison of secretory responses as measured by membrane capacitance and by amperometry.Biophysical journal · 1998Article
- Adenosine triphosphate and the late steps in calcium-dependent exocytosis at a ribbon synapse.The Journal of general physiology · 1998Article
- Ultrastructural organization of bovine chromaffin cell cortex-analysis by cryofixation and morphometry of aspects pertinent to exocytosis.The Journal of cell biology · 1997Article
- A fusion pore phenotype in mast cells of the ruby-eye mouse.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Release of secretory vesicle contents: regulation after fusion?Biophysical journal · 1996Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We measured the exocytotic response induced by flash photolysis of caged compounds in isolated mast cells and chromaffin cells. Vesicle fusion was measured by monitoring the cell membrane capacitance. The release of vesicular contents was followed by amperometry. In response to a GTP gamma S stimulus we found that the time integral of the amperometric current could be superimposed on the capacitance trace. This shows that the integrated amperometric signal provides an alternative method of measuring the extent and kinetics of the secretory response. Very different results were obtained when photolysis of caged Ca2+ (DM-nitrophen) was used to stimulate secretion. In mast cells, there was an immediate, graded increase in membrane capacitance that was followed by step increases (indicative of granule fusion). During the initial phase of the capacitance increases, no release of oxidizable secretory products was detected. In chromaffin cells we also observed a considerable delay between increases in capacitance, triggered by uncaging Ca2+, and the release of oxidizable secretory products. Here we demonstrate that there can be large increases in the membrane capacitance of a secretory cell, triggered by flash photolysis of DM-nitrophen, which indicate events that are not due to the fusion of granules containing oxidizable substances. These results show that increases in capacitance that are not resolved as steps cannot be readily interpreted as secretory events unless they are confirmed independently.
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