ArticleBiophysical journal1996
Measurement of membrane potential and [Ca2+]i in cell ensembles: application to the study of glutamate taste in mice.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 76 citations in OpenAlex.
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Sigma receptors [σRs]: biology in normal and diseased states.Journal of receptor and signal transduction research · 2016Article
- Glutamate: Tastant and Neuromodulator in Taste Buds.Advances in nutrition (Bethesda, Md.) · 2016Review
- Measurement of ATP-Induced Membrane Potential Changes in IVD cells.Cellular and molecular bioengineering · 2014Article
- Genetics of taste receptors.Current pharmaceutical design · 2014Review
- Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2.Biomedical engineering online · 2012Article
- Single-detector simultaneous optical mapping of V(m) and [Ca(2+)](i) in cardiac monolayers.Annals of biomedical engineering · 2012Article
- Umami taste in mice uses multiple receptors and transduction pathways.The Journal of physiology · 2012Article
- Glutamate may be an efferent transmitter that elicits inhibition in mouse taste buds.PloS one · 2012Article
- Tachykinins stimulate a subset of mouse taste cells.PloS one · 2012Article
- Article
- A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation.IEEE transactions on bio-medical engineering · 2011Article
- Article
- Umami responses in mouse taste cells indicate more than one receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Biochemical enrichment and biophysical characterization of a taste receptor for L-arginine from the catfish, Ictalurus puntatus.BMC neuroscience · 2004Article
- Individual mouse taste cells respond to multiple chemical stimuli.The Journal of physiology · 2002Article
- Optical recordings of taste responses from fungiform papillae of mouse in situ.The Journal of physiology · 2001Article
- In situ Ca2+ imaging reveals neurotransmitter receptors for glutamate in taste receptor cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Bitter taste transduction of denatonium in the mudpuppy Necturus maculosus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have studied the spectral properties of the voltage-sensitive dye, 1-(3-sulfonatopropyl)-4-[beta [2-(di-n-octylamino)-6-naphtyl]vinyl] pyridinium betaine (di-8-ANEPPS), and the Ca(2+)-sensitive dye, fura-2, in azolectin liposomes and in isolated taste buds from mouse. We find that the fluorescence excitation spectra of di-8-ANEPPS and fura-2 are largely nonoverlapping, allowing alternate ratio measurements of membrane potential and intracellular calcium ([Ca2+]i). There is a small spillover of di-8-ANEPPS fluorescence at the excitation wavelengths used for fura-2 (340 and 360 nm). However, voltage-induced changes in the fluorescence of di-8-ANEPPS, excited at the fura-2 wavelengths, are small. In addition, di-8-ANEPPS fluorescence is localized to the membrane, whereas fura-2 fluorescence is distributed throughout the cytoplasm. Because of this, the effect of spillover of di-8-ANEPPS fluorescence in the [Ca2+]i estimate is < 1%, under the appropriate conditions. We have applied this method to study of the responses of multiple taste cells within isolated taste buds. We show that membrane potential and [Ca2+]i can be measured alternately in isolated taste buds from mouse. Stimulation with glutamate and glutamate analogs indicates that taste cells express both metabotropic and ionotropic receptors. The data suggest that the receptors responding to 2-amino-4-phosphonobutyrate (L-AP4), presumably metabotropic L-glutamate receptors, do not mediate excitatory glutamate taste responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.