ArticleBiophysical journal1996
Rectification of rabbit cardiac ryanodine receptor current by endogenous polyamines.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Sexual Dimorphism in a Reciprocal Interaction of Ryanodine and IPThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Extensive Ca2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca2+ hypersensitivity.The Journal of general physiology · 2017Article
- Polyamines as Snake Toxins and Their Probable Pharmacological Functions in Envenomation.Toxins · 2016Article
- Polyamines control of cation transport across plant membranes: implications for ion homeostasis and abiotic stress signaling.Frontiers in plant science · 2014Review
- Computational optogenetics: empirically-derived voltage- and light-sensitive channelrhodopsin-2 model.PLoS computational biology · 2013Article
- Article
- Effect of transjunctional KCl gradients on the spermine inhibition of connexin40 gap junctions.Biophysical journal · 2007Article
- Cell membrane-derived lysophosphatidylcholine activates cardiac ryanodine receptor channels.Pflugers Archiv : European journal of physiology · 2007Article
- Amino terminal glutamate residues confer spermine sensitivity and affect voltage gating and channel conductance of rat connexin40 gap junctions.The Journal of physiology · 2004Article
- Ion channels in the outer membranes of chloroplasts and mitochondria: open doors or regulated gates?The EMBO journal · 2001Review
- Amino acid substitutions in the pore of rat glutamate receptors at sites influencing block by polyamines.The Journal of physiology · 1999Article
- Spermine and arcaine block and permeate N-methyl-D-aspartate receptor channels.Biophysical journal · 1999Article
- Imperatoxin A induces subconductance states in Ca2+ release channels (ryanodine receptors) of cardiac and skeletal muscle.The Journal of general physiology · 1998Article
- Interactions of polyamines with ion channels.The Biochemical journal · 1997Review
- Pharmacological modulation of intracellular Ca(2+) channels at the single-channel level.Molecular neurobiologyReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The actions of three endogenous polyamines (spermine, spermidine, and putrescine) were defined on Ca2+ release channels (ryanodine receptors, RyRs) isolated from rabbit cardiac sarcoplasmic reticulum. The current-voltage relationship of the RyR channel was N-shaped in the presence of polyamine (1-5 mM). Polyamine blocked conduction near 0 mV, but the blockade was relieved at large potentials. Polyamines acted (blocked) from both sides of the channel. Polyamine efficacy was dependent on current direction and was inversely related to the ion selectivity of the RyR pore. This suggests that polyamine interacts with current-carrying ions in the permeation pathway. The apparent half-block concentration of spermine at 0 mV was < 0.1 mM. The features of polyamine blockade suggest that the polyamines are permeable cationic blockers of the RyR channel. Further, the levels of polyamines found in muscle cells are sufficient to block single RyR channels and thus may alter the sarcoplasmic reticulum Ca2+ release process in situ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.