ArticleBiophysical journal1996
Response of ryanodine receptor channels to Ca2+ steps produced by rapid solution exchange.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Calcium signaling in postsynaptic mitochondria: mechanisms, dynamics, and role in ATP production.Frontiers in molecular neuroscience · 2025Review
- Loose-patch clamp analysis applied to voltage-gated ionic currents following pharmacological ryanodine receptor modulation in murine hippocampal cornu ammonis-1 pyramidal neurons.Frontiers in physiology · 2024Article
- Cardiac arrhythmogenesis: roles of ion channels and their functional modification.Frontiers in physiology · 2024Review
- Feedback contributions to excitation-contraction coupling in native functioning striated muscle.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- Ryanodine Receptor Activation Induces Long-Term Plasticity of Spine Calcium Dynamics.PLoS biology · 2015Article
- A mechanistic description of gating of the human cardiac ryanodine receptor in a regulated minimal environment.The Journal of general physiology · 2012Article
- Flux regulation of cardiac ryanodine receptor channels.The Journal of general physiology · 2010Article
- Ca(2+) channels on the move.Biochemistry · 2009Review
- Amitriptyline activates cardiac ryanodine channels and causes spontaneous sarcoplasmic reticulum calcium release.Molecular pharmacology · 2009Article
- Calcium regulation of single ryanodine receptor channel gating analyzed using HMM/MCMC statistical methods.The Journal of general physiology · 2004Article
- Markovian models of low and high activity levels of cardiac ryanodine receptors.Biophysical journal · 2001Article
- Ryanodine receptor adaptation.The Journal of general physiology · 2000Review
- Do inactivation mechanisms rather than adaptation hold the key to understanding ryanodine receptor channel gating?The Journal of general physiology · 2000Review
- Rapid activation of the cardiac ryanodine receptor by submillisecond calcium stimuli.The Journal of general physiology · 1999Article
- Ca2+ spark termination: inactivation and adaptation may be manifestations of the same mechanism.The Journal of general physiology · 1999Article
- Caffeine and excitation-contraction coupling in skeletal muscle: a stimulating story.Journal of muscle research and cell motility · 1999Review
- Regulation of cardiac muscle Ca2+ release channel by sarcoplasmic reticulum lumenal Ca2+.Biophysical journal · 1998Article
- Inactivation of Ca2+ release channels (ryanodine receptors RyR1 and RyR2) with rapid steps in [Ca2+] and voltage.Biophysical journal · 1998Article
- Modulation of the frequency of spontaneous sarcoplasmic reticulum Ca2+ release events (Ca2+ sparks) by myoplasmic [Mg2+] in frog skeletal muscle.The Journal of general physiology · 1998Article
- Reduced inhibitory effect of Mg2+ on ryanodine receptor-Ca2+ release channels in malignant hyperthermia.Biophysical journal · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used a flow method for Ca2+ activation of sheep cardiac and rabbit skeletal ryanodine receptor (RyR) channels in lipid bilayers, which activated RyRs in < 20 ms and maintained a steady [Ca2+] for 5 s. [Ca2+] was rapidly altered by flowing Ca(2+)-buffered solutions containing 100 or 200 microM Ca2+ from a perfusion tube inserted in the cis, myoplasmic chamber above the bilayer. During steps from 0.1 to 100 microM, [Ca2+] reached 0.3 microM (activation threshold) and 10 microM (maximum Po) in times consistent with predictions of a solution exchange model. Immediately following rapid RyR activation, Po was 0.67 (cardiac) and 0.45 (skeletal) at a holding voltage of +40 mV (cis/trans). Po then declined (at constant [Ca2+]) in 70% of channels (n = 25) with time constants ranging from .5 to 15 s. The mechanism for Po decline, whether it be adaptation or inactivation, was not determined in this study. cis, 2 mM Mg2+ reduced the initial Po for skeletal RyRs to 0.21 and marginally slowed the declining phase. During very rapid falls in [Ca2+] from mM (inhibited) to sub-microM (sub-activating) levels, skeletal RyR did not open. We conclude the RyR gates responsible for Ca(2+)-dependent activation and inhibition of skeletal RyRs can gate independently.
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