ArticleMolecular & general genetics : MGG1996
Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from a citrus fruit (Citrus unshiu Marc.).
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 53 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analyses reveal biphasic thermal adaptation strategies inPeerJ · 2026Article
- Genome-wide identification and expression profiling analysis of sucrose synthase (SUS) and sucrose phosphate synthase (SPS) genes family in Actinidia chinensis and A. eriantha.BMC plant biology · 2022Article
- Molecular cloning and expression analysis of sucrose phosphate synthase genes in cassava (Manihot esculenta Crantz).Scientific reports · 2020Article
- Investigation of the cause of reduced sugar content in Kiyomi tangor fruit of Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka) rootstock.Scientific reports · 2019Article
- Primary Metabolism in Citrus Fruit as Affected by Its Unique Structure.Frontiers in plant science · 2019Review
- Identification and expression profile analysis of the sucrose phosphate synthase gene family inPeerJ · 2018Article
- Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1).PloS one · 2017Article
- Citrus breeding, genetics and genomics in Japan.Breeding science · 2016Review
- Metabolism of soluble sugars in developing melon fruit: a global transcriptional view of the metabolic transition to sucrose accumulation.Plant molecular biology · 2011Article
- Article
- Citrus genomics.International journal of plant genomics · 2008Article
- Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves.Plant physiology · 2005Article
- Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses.Plant physiology · 2004Article
- Tissue-specific and developmental pattern of expression of the rice sps1 gene.Plant physiology · 2000Article
- Cloning and expression of a prokaryotic sucrose-phosphate synthase gene from the cyanobacterium Synechocystis sp. PCC 6803.Plant molecular biology · 1999Article
- Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.Journal of bacteriology · 1998Article
- Sucrose-phosphate synthase steady-state mRNA increases in ripening kiwifruit.Plant molecular biology · 1998Article
- Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst.Plant physiology · 1997Article
- Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves.Plant physiology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three partial cDNA clones (pSPS1, pSPS2 and pSPS3) encoding sucrose phosphate synthases (SPS) were isolated by Reverse Transcription (RT)-PCR using first-strand cDNA prepared from the leaf or fruit of citrus (Citrus unshiu Marc.). The nucleotide and deduced amino acid sequences of the three clones showed significant similarities to SPS previously isolated in other plants. A full-length, cDNA clone, CitSPS1, was isolated from a fruit (juice sacs and pulp segment) cDNA library using one (pSPS1) of the three partial clones as a probe. The 3539-bp CitSPS1 clone coded for a 1057-amino acid polypeptide with a predicted molecular mass of 117.8 kDa. The amino acid sequence deduced from the CitSPS1 clone showed homology with SPS from maize (55.8% identity) and spinach (74.0% identity). Genomic Southern blot analysis suggested that CitSPS1 clone represents a lowcopy-number gene. RNA blot analysis of leaf, flower and fruit showed that CitSPS1 and pSPS2 were expressed in all organs. However, the levels of expression of CitSPS1 in young leaves, flowers and immature fruits were low, but high in mature leaves, and fruit. pSPS2 transcripts were barely detectable in young leaves and immature fruits, low in mature leaves, and high in flowers and mature fruits. pSPS3 transcripts were only detected in young and in mature leaves.
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