ArticleMolecular & general genetics : MGG1996
The role of the genes nrf EFG and ccmFH in cytochrome c biosynthesis in Escherichia coli.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- From genes to function: regulation, maturation, and evolution of cytochromeApplied and environmental microbiology · 2025Review
- Vibrio natriegens as a superior host for the production of c-type cytochromes and difficult-to-express redox proteins.Scientific reports · 2024Article
- Genetic Dissection of the Fermentative and Respiratory Contributions Supporting Vibrio cholerae Hypoxic Growth.Journal of bacteriology · 2020Article
- Structure of a DsbF homologue from Corynebacterium diphtheriae.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Structural and biochemical characterization of the essential DsbA-like disulfide bond forming protein from Mycobacterium tuberculosis.BMC structural biology · 2013Article
- Cytochrome c biogenesis System I.The FEBS journal · 2011Review
- An extracellular disulfide bond forming protein (DsbF) from Mycobacterium tuberculosis: structural, biochemical, and gene expression analysis.Journal of molecular biology · 2010Article
- The Pasteurella multocida nrfE gene is upregulated during infection and is essential for nitrite reduction but not for virulence.Journal of bacteriology · 2005Article
- The roles of different regions of the CycH protein in c-type cytochrome biogenesis in Sinorhizobium meliloti.Molecular genetics and genomics : MGG · 2004Article
- Variation of the axial haem ligands and haem-binding motif as a probe of the Escherichia coli c-type cytochrome maturation (Ccm) system.The Biochemical journal · 2003Article
- Periplasmic nitrate reductase (NapABC enzyme) supports anaerobic respiration by Escherichia coli K-12.Journal of bacteriology · 2002Article
- In vivo-expressed genes of Pasteurella multocida.Infection and immunity · 2001Article
- The Escherichia coli CcmG protein fulfils a specific role in cytochrome c assembly.The Biochemical journal · 2001Article
- Comparison of the Escherichia coli K-12 genome with sampled genomes of a Klebsiella pneumoniae and three salmonella enterica serovars, Typhimurium, Typhi and Paratyphi.Nucleic acids research · 2000Article
- Integrity of thermus thermophilus cytochrome c552 synthesized by Escherichia coli cells expressing the host-specific cytochrome c maturation genes, ccmABCDEFGH: biochemical, spectral, and structural characterization of the recombinant protein.Protein science : a publication of the Protein Society · 2000Article
- Transcriptional control and essential roles of the Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis.The Biochemical journal · 1998Article
- Linkage map of Escherichia coli K-12, edition 10: the traditional map.Microbiology and molecular biology reviews : MMBR · 1998Review
- Cell biology and molecular basis of denitrification.Microbiology and molecular biology reviews : MMBR · 1997Review
- Biogenesis of respiratory cytochromes in bacteria.Microbiology and molecular biology reviews : MMBR · 1997Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been suggested that two groups of Escherichia coli genes, the ccm genes located in the 47-min region and the nrfEFG genes in the 92-min region of the chromosome, are involved in cytochrome c biosynthesis during anaerobic growth. The involvement of the products of these genes in cytochrome c synthesis, assembly and secretion has now been investigated. Despite their similarity to other bacterial cytochrome c assembly proteins, NrfE, F and G were found not to be required for the biosynthesis of any of the c-type cytochromes in E. coli. Furthermore, these proteins were not required for the secretion of the periplasmic cytochromes, cytochrome C550 and cytochrome C552, or for the correct targeting of the NapC and NrfB cytochromes to the cytoplasmic membrane. NrfE and NrfG are required for formate-dependent nitrite reduction (the Nrf pathway), which involves at least two c-type cytochromes, cytochrome C552 and NrfB, but NrfF is not essential for this pathway. Genes similar to nrfE, nrfF and nrfG are present in the E. coli nap-ccm locus at minute 47. CcmF is similar to NrfE, the N-terminal region of CcmH is similar to NrfF and the C-terminal portion of CcmH is similar to NrfG. In contrast to NrfF, the N-terminal, NrfF-like portion of CcmH is essential for the synthesis of all c-type cytochromes. Conversely, the NrfG-like C-terminal region of CcmH is not essential for cytochrome c biosynthesis. The data are consistent with proposals from this and other laboratories that CcmF and CcmH form part of a haem lyase complex required to attach haem c to C-X-X-C-H haem-binding domains. In contrast, NrfE and NrfG are proposed to fulfill a more specialised role in the assembly of the formate-dependent nitrite reductase.
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