ArticleThe Journal of physiology1996
On muscarinic control of neurogenic mucus secretion in ferret trachea.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 82 citations in OpenAlex.
- Airway mucus in infection.Frontiers in physiology · 2026Review
- Physiology and pathophysiology of mucus and mucolytic use in critically ill patients.Critical care (London, England) · 2025Review
- Review
- Lung innervation in the eye of a cytokine storm: neuroimmune interactions and COVID-19.Nature reviews. Neurology · 2020Review
- The mode of action of anticholinergics in asthma.The European respiratory journal · 2018Review
- Mechanisms of organophosphorus pesticide toxicity in the context of airway hyperreactivity and asthma.American journal of physiology. Lung cellular and molecular physiology · 2018Review
- Feishu Acupuncture Inhibits Acetylcholine Synthesis and Restores Muscarinic Acetylcholine Receptor M2 Expression in the Lung When Treating Allergic Asthma.Inflammation · 2018Article
- Pleiotropic effects and pharmacological properties of penehyclidine hydrochloride.Drug design, development and therapy · 2018Review
- Mechanisms of airway responses to esophageal acidification in cats.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- How does rhinovirus cause the common cold cough?BMJ open respiratory research · 2016Article
- Bencycloquidium bromide inhibits nasal hypersecretion in a rat model of allergic rhinitis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2015Article
- Crosstalk between beta-2-adrenoceptor and muscarinic acetylcholine receptors in the airway.Current opinion in pharmacology · 2014Review
- Long-acting muscarinic receptor antagonists for the treatment of chronic airway diseases.Therapeutic advances in chronic disease · 2014Review
- Pharmacogenetics of asthma controller treatment.The pharmacogenomics journal · 2013Article
- The role of tiotropium in the management of asthma.Asia Pacific allergy · 2012Article
- Muscarinic receptor antagonists: effects on pulmonary function.Handbook of experimental pharmacology · 2012Review
- Mucus secretion from individual submucosal glands of the ferret trachea.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Mucoactive drugs.European respiratory review : an official journal of the European Respiratory Society · 2010Review
- Tiotropium bromide: an update.The open respiratory medicine journal · 2009Article
- Potential adverse effects of bronchodilators in the treatment of airways obstruction in older people: recommendations for prescribing.Drugs & aging · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Muscarinic receptor subtypes mediating neurogenic mucus secretion in ferret trachea were characterized in vitro and in vivo using 35SO4 as a label for secreted mucus, and the muscarinic receptor antagonists telenzepine for the M1 receptor subtype, methoctramine for the M2 subtype and 4-diphenylacetoxy-N-methylpiperidine methobromide (4-DAMP) for the M3 receptor. We also performed receptor binding and mapping studies. 2. Each muscarinic antagonist displaced [N-methyl-3H]scopolamine binding with high-affinity binding constant (KH) values of 1.9, 2.7 and 5.0 nM for telenzepine, methoctramine and 4-DAMP, respectively. Muscarinic M1 and M3 receptors localized to submucosal glands, whereas M2 receptors did not. 3. In vitro, electrical stimulation (50 V, 10 Hz, 0.5 ms for 5 min) increased 35SO4 output by 160%. Telenzepine did not inhibit the neurogenic secretory response at concentrations two-or twentyfold its KH value, nor did it inhibit secretion induced by acetylcholine (ACh). 4-DAMP inhibited neurogenic secretion by 80 and 95%, respectively, at concentrations two-and twentyfold its KH value, and also inhibited ACh-induced secretion. Methoctramine potentiated neurogenic secretion induced at 2.5 Hz (50 V, 0.5 ms for 5 min) in a dose-related (5.4-100 nM) manner with increases of 33-451% above electrically stimulated values. Methoctramine did not potentiate secretion induced at 10 Hz and did not have any effect on ACh-induced secretion. 4. In vivo, vagal stimulation (10 V, 10 Hz, 2 ms for 8 min) increased output of 35SO4 by approximately 120%. Telenzepine had no significant effect on neurogenic secretion. Methoctramine approximately doubled the stimulated response, whereas 4-DAMP abolished the stimulated secretory response. 5. We conclude that in ferret trachea, cholinergic nerve stimulation increases mucus secretion via muscarinic M3 receptors on the submucosal glands. The magnitude of the secretory response is regulated by neuronal M2 muscarinic receptors. The muscarinic M1 receptors localized to the submucosal glands do not appear to be involved with mucus secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.