ArticleJournal of cell science1996
Initiation and maintenance of NGF-stimulated neurite outgrowth requires activation of a phosphoinositide 3-kinase.
Article in Journal of cell science, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 126 citations in OpenAlex.
- Protein kinases: master regulators of neuritogenesis and therapeutic targets for axon regeneration.Cellular and molecular life sciences : CMLS · 2020Review
- UBTOR/KIAA1024 regulates neurite outgrowth and neoplasia through mTOR signaling.PLoS genetics · 2018Article
- 6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells.BMC complementary and alternative medicine · 2017Article
- Cytoskeletal and signaling mechanisms of neurite formation.Cell and tissue research · 2015Review
- Potentiation of neuritogenic activity of medicinal mushrooms in rat pheochromocytoma cells.BMC complementary and alternative medicine · 2013Article
- Regulation of PI-3-Kinase and Akt Signaling in T Lymphocytes and Other Cells by TNFR Family Molecules.Frontiers in immunology · 2013Article
- Ras effector switching promotes divergent cell fates in C. elegans vulval patterning.Developmental cell · 2011Article
- Akt regulates the expression of MafK, synaptotagmin I, and syntenin-1, which play roles in neuronal function.Journal of biomedical science · 2010Article
- Article
- Functional whole-genome analysis identifies Polo-like kinase 2 and poliovirus receptor as essential for neuronal differentiation upstream of the negative regulator alphaB-crystallin.The Journal of biological chemistry · 2009Article
- Involvement of Akt in neurite outgrowth.Cellular and molecular life sciences : CMLS · 2009Review
- Insulin and insulin-like growth factor resistance in alcoholic neurodegeneration.Alcoholism, clinical and experimental research · 2008Article
- Nerve growth factor potentiates p53 DNA binding but inhibits nitric oxide-induced apoptosis in neuronal PC12 cells.Neurochemical research · 2007Article
- Role of Src family kinases and N-Myc in spermatogonial stem cell proliferation.Developmental biology · 2007Article
- The Ras-GRF1 exchange factor coordinates activation of H-Ras and Rac1 to control neuronal morphology.Molecular biology of the cell · 2006Article
- The inositol polyphosphate 5-phosphatase, PIPP, Is a novel regulator of phosphoinositide 3-kinase-dependent neurite elongation.Molecular biology of the cell · 2006Article
- Critical role for Kalirin in nerve growth factor signaling through TrkA.Molecular and cellular biology · 2005Article
- Second-generation antipsychotic drugs, olanzapine, quetiapine, and clozapine enhance neurite outgrowth in PC12 cells via PI3K/AKT, ERK, and pertussis toxin-sensitive pathways.Journal of molecular neuroscience : MN · 2005Article
- Inhibition of neuronal phenotype by PTEN in PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Role of integrin-linked kinase in nerve growth factor-stimulated neurite outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Application of nerve growth factor (NGF) to PC12 cells stimulates a programme of physiological changes leading to the development of a sympathetic neuron like phenotype, one aspect of which is the development of a neuronal morphology characterised by the outgrowth of neuritic processes. We have investigated the role of phosphoinositide 3-kinase in NGF-stimulated morphological differentiation through two approaches: firstly, preincubation with wortmannin, a reputedly specific inhibitor of phosphoinositide kinases, completely inhibited initial morphological responses to NGF, the formation of actin filament rich microspikes and subsequent neurite outgrowth. This correlated with wortmannin inhibition of NGF-stimulated phosphatidylinositol(3,4,5)trisphosphate (PtdInsP3) and phosphatidylinositol(3,4)bisphosphate (PtdIns(3,4)P2) production and with inhibition of NGF-stimulated phosphoinositide 3-kinase activity in anti-phosphotyrosine immunoprecipitates. Secondly, the overexpression of a mutant p85 regulatory subunit of the phosphoinositide 3-kinase, which cannot interact with the catalytic p110 subunit, also substantially inhibited the initiation of NGF-stimulated neurite outgrowth. In addition, we found that wortmannin caused a rapid collapse of more mature neurites formed following several days exposure of PC12 cells to NGF. These results indicate that NGF-stimulated neurite outgrowth requires the activity of a tyrosine kinase regulated PI3-kinase and suggest that the primary product of this enzyme, PtdInsP3, is a necessary second messenger for the cytoskeletal and membrane reorganization events which occur during neuronal differentiation.
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