ArticleThe Plant cell1996
Targeting and processing of a chimeric protein with the N-terminal presequence of the precursor to glyoxysomal citrate synthase.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 73 citations in OpenAlex.
- A redox-regulated RCC1-like protein controls catalase activity in Arabidopsis.Nature communications · 2026Article
- Ubiquitin-conjugating activity by PEX4 is required for efficient protein transport to peroxisomes in Arabidopsis thaliana.The Journal of biological chemistry · 2022Article
- Nuclear and peroxisomal targeting of catalase.Plant, cell & environment · 2022Article
- Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile AlgaFrontiers in cell and developmental biology · 2020Article
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- Article
- Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15 and contributes to normal peroxisome metabolism.Plant molecular biology · 2013Article
- Proteomic analysis reveals that the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEX7 (peroxin 7).The Journal of biological chemistry · 2013Article
- Article
- Peroxisomal targeting signals in green algae.Protoplasma · 2009Article
- Peroxisome biogenesis and function.The arabidopsis book · 2009Article
- The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant physiology · 2008Article
- Fluorescent co-localization of PTS1 and PTS2 and its application in analysis of the gene function and the peroxisomal dynamic in Magnaporthe oryzae.Journal of Zhejiang University. Science. B · 2008Article
- Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination.The Plant cell · 2005Article
- Specification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses.Plant physiology · 2004Article
- Import of the peroxisomal targeting signal type 2 protein 3-ketoacyl-coenzyme a thiolase into glyoxysomes.Plant physiology · 2003Article
- Molecular characterization of an Arabidopsis acyl-coenzyme a synthetase localized on glyoxysomal membranes.Plant physiology · 2002Article
- AtPex14p maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes.The EMBO journal · 2000Article
- Caenorhabditis elegans has a single pathway to target matrix proteins to peroxisomes.EMBO reports · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glyoxysomal citrate synthase in pumpkin is synthesized as a precursor that has a cleavable presequence at its N-terminal end. To investigate the role of the presequence in the transport of the protein to the microbodies, we generated transgenic Arabidopsis plants that expressed beta-glucuronidase with the N-terminal presequence of the precursor to the glyoxysomal citrate synthase of pumpkin. Immunogold labeling and cell fractionation studies showed that the chimeric protein was transported into microbodies and subsequently was processed. The chimeric protein was transported to functionally different microbodies, such as glyoxysomes, leaf peroxisomes, and unspecialized microbodies. These observations indicated that the transport of glyoxysomal citrate synthase is mediated by its N-terminal presequence and that the transport system is functional in all plant microbodies. Site-directed mutagenesis of the conserved amino acids in the presequence caused abnormal targeting and inhibition of processing of the chimeric protein, suggesting that the conserved amino acids in the presequence are required for recognition of the target or processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.