ArticleThe Biochemical journal1996
High rates of extracellular superoxide generation by cultured human fibroblasts: involvement of a lipid-metabolizing enzyme.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 75 citations in OpenAlex.
- Regulation of pathophysiological and tissue regenerative functions of MSCs mediated via the WNT signaling pathway (Review).Molecular medicine reports · 2021Review
- Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo.Journal of cellular and molecular medicine · 2018Review
- Regulation of neuronal development and function by ROS.FEBS letters · 2018Review
- Chemical composition and phagocyte immunomodulatory activity ofJournal of leukocyte biology · 2017Article
- The role of reactive oxygen species in mesenchymal stem cell adipogenic and osteogenic differentiation: a review.Stem cells and development · 2015Review
- Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases.Physiological reviews · 2014Review
- OXPHOS mutations and neurodegeneration.The EMBO journal · 2013Review
- Oleic, linoleic and linolenic acids increase ros production by fibroblasts via NADPH oxidase activation.PloS one · 2013Article
- Plasma membrane electron transport in pancreatic β-cells is mediated in part by NQO1.American journal of physiology. Endocrinology and metabolism · 2011Article
- Thiol chemistry in peroxidase catalysis and redox signaling.Antioxidants & redox signaling · 2008Review
- Mechanisms underlying caloric restriction and lifespan regulation: implications for vascular aging.Circulation research · 2008Review
- Kinetic modeling of nitric-oxide-associated reaction network.Pharmaceutical research · 2006Article
- The plasma membrane redox system: a candidate source of aging-related oxidative stress.Age (Dordrecht, Netherlands) · 2005Article
- Vibrio fischeri genes hvnA and hvnB encode secreted NAD(+)-glycohydrolases.Journal of bacteriology · 2001Article
- Superoxide generation of phagocytes and nonphagocytic cells.Protoplasma · 2001Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of NADPH oxidase and low superoxide generation (approx. 0.06 nmol/min per 10(6) cells) by cytokine- or ionophore-stimulated human fibroblasts is known. However, we here show that these cells also contain an ectoplasmic enzyme, distinct from NADPH oxidase, which can generate superoxide (2.19 +/- 0.14 nmol/min per 10(6) cells) at levels similar to phorbol ester-stimulated monocytes on exogenous NADH addition. Superoxide generation was temperature-dependent, insensitive to chelation (desferal), and had a K(m) (app)(NADH) of 11.5 microM. Inhibitor studies showed that there was no involvement of NADPH oxidase (diphenylene iodonium, diphenyl iodonium), prostaglandin H synthase (indomethacin), xanthine oxidase (allopurinol), cytochrome P-450 (metyrapone) or mitochondrial respiration (rotenone, antimycin A). NAD+ was a competitive inhibitor, whereas NADPH supported 40% of the rate seen with NADH. No luminescence was observed after the addition of lactate, malate, pyruvate, GSH or L-cysteine. NADH-stimulated superoxide generation was enhanced by the addition of (3-30 microM) arachidonic acid, linoleic acid or (5S)-hydroxyeicosatetraenoic acid [(5S)-HETE] but not palmitic acid, (15S)-hydroperoxyeicosatetraenoic acid [(15S)-HPETE], (15S)-HETE or (12S)-HETE. Several features suggest involvement of an enzyme related to 15-lipoxygenase, and, in support of this, we show superoxide generation and NADH oxidation by recombinant rabbit reticulocyte 15-lipoxygenase. The large amounts of superoxide measured suggest that the fibroblast extracellular enzyme could be a major source of reactive oxygen species after tissue damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.