ArticleMammalian genome : official journal of the International Mammalian Genome Society1996
Homologous and heterologous FISH painting with PARM-PCR chromosome-specific probes in mammals.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 citing papers in PubMed, 14 citations in OpenAlex.
- Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17).PloS one · 2013Article
- Quantitative fluorescence in situ hybridization in lung cancer as a diagnostic marker.The Journal of molecular diagnostics : JMD · 1999Article
- Comparative karyotype of rat and mouse using bidirectional chromosome painting.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
- Characterization of reciprocal translocations in pigs using dual-colour chromosome painting and primed in situ DNA labelling.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous loci can be amplified by PARM-PCR on 300 sorted chromosomes in low-stringency conditions (annealing at 30 degrees C during the two first cycles) to produce a probe that can be used in FISH painting experiments. We demonstrate that, depending on the primer chosen for the amplification, patterns of different quality can be obtained. In order to design a primer that allows amplification of coding sequences, we have shown that motifs of at least seven glutamic acid repeats (GAG or GAA codons) are present in human proteins more frequently than expected. Moreover, these repeats do not correspond to triplet expansion and can be conserved between species. Using probes prepared from sorted chromosomes with (GAG)7 primer, we were able to achieve homologous FISH painting on human, porcine, ovine, and bovine species, and bidirectional heterologous FISH painting between human and porcine species. As an example, using probes for human Chromosome (Chr) 19 and porcine Chrs 1 and 6, we clearly defined the regional homologies existing between those chromosomes.
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