ArticleThe Journal of cell biology1996
Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix.Frontiers in cell and developmental biology · 2018Article
- Mast Cell Mediators Inhibit Osteoblastic Differentiation and Extracellular Matrix Mineralization.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- The FcεRI Signaling Cascade and Integrin Trafficking Converge at Patterned Ligand Surfaces.Molecular biology of the cell · 2017Article
- Graphene Oxide Nanosheets Stimulate Ruffling and Shedding of Mammalian Cell Plasma Membranes.Chem · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- Orthogonal patterning of multiple biomolecules using an organic fluorinated resist and imprint lithography.Biomacromolecules · 2013Article
- Roles for ca(2+) mobilization and its regulation in mast cell functions.Frontiers in immunology · 2012Article
- Ca2+ waves initiate antigen-stimulated Ca2+ responses in mast cells.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cholesterol-dependent separation of the beta2-adrenergic receptor from its partners determines signaling efficacy: insight into nanoscale organization of signal transduction.The Journal of biological chemistry · 2008Article
- Real-time cross-correlation image analysis of early events in IgE receptor signaling.Biophysical journal · 2008Article
- Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles.Biochimica et biophysica acta · 2008Article
- Fluorescence resonance energy transfer between lipid probes detects nanoscopic heterogeneity in the plasma membrane of live cells.Biophysical journal · 2007Article
- Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Membrane order and molecular dynamics associated with IgE receptor cross-linking in mast cells.Biophysical journal · 2007Article
- Endoplasmic reticulum architecture: structures in flux.Current opinion in cell biology · 2006Review
- Coexisting domains in the plasma membranes of live cells characterized by spin-label ESR spectroscopy.Biophysical journal · 2006Article
- Visualization of plasma membrane compartmentalization with patterned lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosis.Molecular biology of the cell · 2004Article
- Quantitative analysis of the fluorescence properties of intrinsically fluorescent proteins in living cells.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cells of the mucosal mast cell line, RBL-2H3, are normally stimulated to degranulate after aggregation of high affinity receptors for IgE (Fc epsilon RI) by soluble cross-linking ligands. This cellular degranulation process requires sustained elevation of cytoplasmic Ca2+. In this study, we investigated the response of RBL-2H3 cells to 6-micron beads coated with IgE-specific ligands. These ligand-coated beads cause only small, transient Ca2+ responses, even though the same ligands added in soluble form cause larger, more sustained Ca2+ responses. The ligand-coated 6-micron beads also fail to stimulate significant degranulation of RBL-2H3 cells, whereas much larger ligand-coated Sepharose beads stimulate ample degranulation. Confocal fluorescence microscopy shows that the 6-micron beads (but not the Sepharose beads) are phagocytosed by RBL-2H3 cells and that, beginning with the initial stages of bead engulfment, there is exclusion of many plasma membrane components from the 6-micron bead/cell interface, including p53/56lyn and several other markers for detergent-resistant membrane domains, as well as an integrin and unliganded IgE-Fc epsilon RI. The fluorescent lipid probe DiIC16 is a marker for the membrane domains that is excluded from the cell/bead interface, whereas a structural analogue, fast DiI, which differs from DiIC16 by the presence of unsaturated acyl chains, is not substantially excluded from the interface. None of these components are excluded from the interface of RBL-2H3 cells and the large Sepharose beads. Additional confocal microscopy analysis indicates that microfilaments are involved in the exclusion of plasma membrane components from the cell/bead interface. These results suggest that initiation of phagocytosis diverts normal signaling pathways in a cytoskeleton-driven membrane clearance process that alters the physiological response of the cells.
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